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Optimization of Agrobacterium-mediated transformation parameters for sweet potato embryogenic callus using -glucuronidase (GUS) as a reporter

Xing YuJun, Yang Qing, , Luo Yuming, Zhang Yunfeng, Gu Ke, Wang Dengzhan
Published: 19 November 2007

Abstract: Agrobacterium-mediated transformation factors for sweet potato embryogenic calli were optimized using β-glucuronidase (GUS) as a reporter. The binary vector pTCK303 harboring the modified GUS gene driven by the CaMV 35S promoter was used. Transformation parameters were optimized including bacterial concen-tration, pre-culture period, co-cultivation period, immersion time, acetosyringone (AS) concentration and mannitol treated time. Results were obtained based on the percentage of GUS expression. Agrobacterium tumefaciens strain EHA105 at concentration OD600 nm = 0.8 showed the highest virulence on sweet potato embryogenic callus. Four days of pre-culture, four days of co-cultivation, 10 min of immersion, 200 μM acetosyringone and 60 min of mannitol-treated embryogenic callus gave the highest percentage of GUS positive transformants. Key words: Agrobacterium-mediated, transformation parameters, sweet potato embryogenic callus, β-glucuronidase.
Keywords: -mediated / Transformation Parameters / Sweet Potato Embryogenic Callus / -glucuronidase.

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