Myostatin in the Arterial Wall of Patients with End-Stage Renal Disease
Open Access
- 1 October 2020
- journal article
- research article
- Published by Japan Atherosclerosis Society in Journal of Atherosclerosis and Thrombosis
- Vol. 27 (10), 1039-1052
- https://doi.org/10.5551/jat.51144
Abstract
Aim: Myostatin (Mstn) has been described as a trigger for the progression of atherosclerosis. In this study, we evaluated the role of Mstn in arterial remodeling in patients with end-stage renal disease (ESRD). Methods: Vascular specimens were collected from 16 ESRD patients (56.4±7.9 years) undergoing renal transplant (recipients) and 15 deceased kidney non-uremic donors (55.4±12.1 years). We studied gene and protein expression of Mstn, ubiquitin ligases, Atrogin-1, and muscle ring finger protein-1 (MuRF-1), inflammatory marker CCL2, cytoskeleton components, and Klotho by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. Moreover, we assessed vascular calcification and collagen deposition. Finally, we studied the effects of recombinant Mstn on rat vascular smooth muscle cells (VSMCs, A7r5) and evaluated the effects of uremic serum (US) on primary human VSMCs. Results: Myostatin mRNA was upregulated in the arterial vascular wall of recipients compared with donors (~15- folds, p<0.05). This response was accompanied by the upregulation of gene expression of Atrogin-1 and MuRF-1 (+2.5- and +10-fold) and CCL2 (+3-fold). Conversely, we found downregulation of protein expression of Smoothelin, α-smooth muscle actin (α-SMA), vimentin, and Klotho (-85%, -50%, -70%, and -80%, respectively; p<0.05) and gene expression of vimentin and Klotho. Exposition of A7r5 to Mstn induced a time-dependent SMAD 2/SMAD 3 phosphorylation and expression of collagen-1 and transforming growth factor β (TGFβ) mRNA, while US induced overexpression of Mstn and Atrogin-1 and downregulation of Smoothelin and Klotho. Conclusions: Our data suggest that uremia might induce vascular Mstn gene expression together with a complex pathway of molecular and structural changes in the vascular wall. Myostatin, in turn, can translate the metabolic alterations of uremia into profibrotic and stiffness inducing signals.Keywords
This publication has 53 references indexed in Scilit:
- Stat3 Activation Links a C/EBPδ to Myostatin Pathway to Stimulate Loss of Muscle MassCell Metabolism, 2013
- Abdominal aortic calcification is not superior over other vascular calcification in predicting mortality in hemodialysis patients: a retrospective observational studyBMC Nephrology, 2013
- Arterial Klotho Expression and FGF23 Effects on Vascular Calcification and FunctionPLOS ONE, 2013
- Aging Enhances the Basal Production of IL-6 and CCL2 in Vascular Smooth Muscle CellsArteriosclerosis, Thrombosis, and Vascular Biology, 2012
- Klotho Deficiency Causes Vascular Calcification in Chronic Kidney DiseaseJournal of the American Society of Nephrology, 2011
- Superoxide dismutase type 1 in monocytes of chronic kidney disease patientsAmino Acids, 2010
- Cardiac Muscle Ring Finger-1 Increases Susceptibility to Heart Failure In VivoCirculation Research, 2009
- Mechanisms of Vascular Calcification in Chronic Kidney DiseaseJournal of the American Society of Nephrology, 2008
- Atrogin-1 inhibits Akt-dependent cardiac hypertrophy in mice via ubiquitin-dependent coactivation of Forkhead proteinsJCI Insight, 2007
- Downregulation of SnoN Expression in Obstructive Nephropathy Is Mediated by an Enhanced Ubiquitin-Dependent DegradationJournal of the American Society of Nephrology, 2006