ZSCAN 4 + mouse embryonic stem cells have an oxidative and flexible metabolic profile
- 18 May 2020
- journal article
- research article
- Published by EMBO in EMBO Reports
- Vol. 21 (6), e48942
- https://doi.org/10.15252/embr.201948942
Abstract
Cultured mouse embryonic stem cells are a heterogeneous population with diverse differentiation potential. In particular, the subpopulation marked by Zscan4 expression has high stem cell potency and shares with 2 cell stage preimplantation embryos both genetic and epigenetic mechanisms that orchestrate zygotic genome activation. Although embryonic de novo genome activation is known to rely on metabolites, a more extensive metabolic characterization is missing. Here we analyze the Zscan4+ mouse stem cell metabolic phenotype associated with pluripotency maintenance and cell reprogramming. We show that Zscan4+ cells have an oxidative and adaptable metabolism, which, on one hand, fuels a high bioenergetic demand and, on the other hand, provides intermediate metabolites for epigenetic reprogramming. Our findings enhance our understanding of the metastable Zscan4+ stem cell state with potential applications in regenerative medicine.Keywords
Funding Information
- Consiglio Nazionale delle Ricerche (Interomics2017)
- Ministero dell’Istruzione, dell’Università e della Ricerca (2017XJ38A4)
- Università degli Studi di Napoli Federico II (STAR Linea 1‐ 2014)
This publication has 36 references indexed in Scilit:
- Identification of a Novel Gene Signature of ES Cells Self-Renewal Fluctuation through System-Wide AnalysisPLOS ONE, 2014
- Telomere Length Reprogramming in Embryos and Stem CellsBioMed Research International, 2014
- A role for polyamine regulators in ESC self-renewalCell Cycle, 2012
- Zscan4 promotes genomic stability during reprogramming and dramatically improves the quality of iPS cells as demonstrated by tetraploid complementationCell Research, 2012
- Redox regulation of mitochondrial biogenesisFree Radical Biology & Medicine, 2012
- RYBP Represses Endogenous Retroviruses and Preimplantation- and Germ Line-Specific Genes in Mouse Embryonic Stem CellsMolecular and Cellular Biology, 2012
- Low concentrations of nitric oxide delay the differentiation of embryonic stem cells and promote their survivalCell Death & Disease, 2010
- Zscan4 regulates telomere elongation and genomic stability in ES cellsNature, 2010
- Maternal control of early mouse developmentDevelopment, 2010
- RNA transcript profiling during zygotic gene activation in the preimplantation mouse embryoDevelopmental Biology, 2005