Achieving stable myocardial regeneration after apical resection in neonatal mice
Open Access
- 1 June 2020
- journal article
- research article
- Published by Wiley in Journal of Cellular and Molecular Medicine
- Vol. 24 (11), 6500-6504
- https://doi.org/10.1111/jcmm.15223
Abstract
The neonatal heart completely regenerates after apical resection (AR), providing a desirable research model to study the mechanism of cardiac regeneration and cardiomyocyte proliferation. However, AR-induced neonatal heart regenerative phenomenon is controversial due to the variation of operative details in different laboratories. Here, we provide an optimized AR operation procedure with stable regeneration and high survival rate by achieving heart exposure, normalizing myocardium cut-offs, and reducing operation duration. We also established a whole-heart-slice approach to estimate the myocardial regeneration after the AR operation, which ensures no false-negative/positive results. The combination of the optimized AR operation and the whole-heart-slice analysis provides a stable system to study neonatal heart regeneration and cardiomyocyte proliferation in situ.Funding Information
- CAMS Innovation Fund for Medical Sciences (2016-I2M-1-015)
- National Key Research and Development Project of China (2019YFA0801500)
- National Natural Science Foundation of China (81970243, 81770308)
- Beijing Natural Science Foundation (7172183)
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