Transcriptional Differences between Canine Cutaneous Epitheliotropic Lymphoma and Immune-Mediated Dermatoses
Open Access
- 25 January 2021
- Vol. 12 (2), 160
- https://doi.org/10.3390/genes12020160
Abstract
Canine cutaneous epitheliotropic T-cell lymphoma (CETL) and immune-mediated T-cell predominant dermatoses (IMD) share several clinical and histopathological features, but differ substantially in prognosis. The discrimination of ambiguous cases may be challenging, as diagnostic tests are limited and may prove equivocal. This study aimed to investigate transcriptional differences between CETL and IMD, as a basis for further research on discriminating diagnostic biomarkers. We performed 100bp single-end sequencing on RNA extracted from formalin-fixed and paraffin-embedded skin biopsies from dogs with CETL and IMD, respectively. DESeq2 was used for principal component analysis (PCA) and differential gene expression analysis. Genes with significantly different expression were analyzed for enriched pathways using two different tools. The expression of selected genes and their proteins was validated by RT-qPCR and immunohistochemistry. PCA demonstrated the distinct gene expression profiles of CETL and IMD. In total, 503 genes were upregulated, while 4986 were downregulated in CETL compared to IMD. RT-qPCR confirmed the sequencing results for 5/6 selected genes tested, while the protein expression detected by immunohistochemistry was not entirely consistent. Our study revealed transcriptional differences between canine CETL and IMD, with similarities to human cutaneous lymphoma. Differentially expressed genes are potential discriminatory markers, but require further validation on larger sample collections.Funding Information
- European Society of Veterinary Dermatology (ESVD Major Grant 2018)
This publication has 66 references indexed in Scilit:
- STAR: ultrafast universal RNA-seq alignerBioinformatics, 2012
- Primer3—new capabilities and interfacesNucleic Acids Research, 2012
- Measuring dementia carers' unmet need for services - an exploratory mixed method studyBMC Health Services Research, 2010
- Transcriptional Profiles Predict Disease Outcome in Patients with Cutaneous T-Cell LymphomaClinical Cancer Research, 2010
- Paucity of intraepidermal FoxP3‐positive T cells in cutaneous T‐cell lymphoma in contrast with spongiotic and lichenoid dermatitisJournal of Cutaneous Pathology, 2010
- Cutaneous T-Cell Lymphoma: Roles for Chemokines and Chemokine ReceptorsJournal of Investigative Dermatology, 2009
- Lesional gene expression profiling in cutaneous T-cell lymphoma reveals natural clusters associated with disease outcomeBlood, 2007
- Identification of new reference genes for the normalisation of canine osteoarthritic joint tissue transcripts from microarray dataBMC Molecular Biology, 2007
- KOBAS server: a web-based platform for automated annotation and pathway identificationNucleic Acids Research, 2006
- Integrin signalling during tumour progressionNature Reviews Molecular Cell Biology, 2004