Decreased grain size1, a C3HC4-type RING protein, influences grain size in rice (Oryza sativa L.)
- 2 January 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Plant Molecular Biology
- Vol. 105 (4-5), 405-417
- https://doi.org/10.1007/s11103-020-01096-7
Abstract
Key message We reported that DGS1 plays a positive role in regulating grain size in rice and was regulated by OsBZR1. Abstract Grain size is an important agronomic trait that contributes to grain yield. However, the underlying molecular mechanisms that determine final grain size are still largely unknown. We isolated a rice mutant showing reduced grain size in a 60Co-irradiated variety Nanjing 35 population. We named the mutant decreased grain size1 (dgs1). Map-based cloning and subsequent transgenic CRISPR and complementation assays indicated that a mutation had occurred in LOC_Os03g49900 and that the DGS1 allele regulated grain size. DGS1 encodes a protein with a 7-transmembrane domain and C3HC4 type RING domain. It was widely expressed, especially in young tissues. DGS1 is a membrane-located protein. OsBZR1 (BRASSINAZOLE-RESISTANT1), a core transcription activator of BR signaling, also plays a positive role in grain size. We provided preliminary evidence that OsBZR1 can bind to the DGS1 promoter to activate expression of DGS1.Keywords
Funding Information
- Jiangsu Provincial Key Research and Development Program (2017YFD0100404)
- Jiangsu Key Laboratory of Photonic Manufacturing Science and Technology (BE2018388)
- Key Project for Jiangsu Agricultural New Variety Innovation (PZCZ201701)
This publication has 48 references indexed in Scilit:
- Rice qGL3/OsPPKL1 Functions with the GSK3/SHAGGY-Like Kinase OsGSK3 to Modulate Brassinosteroid SignalingPlant Cell, 2019
- OsSHI1 Regulates Plant Architecture Through Modulating the Transcriptional Activity of IPA1 in RiceTHE PLANT CELL ONLINE, 2019
- Ubiquitylation activates a peptidase that promotes cleavage and destabilization of its activating E3 ligases and diverse growth regulatory proteins to limit cell proliferation in ArabidopsisGenes & Development, 2017
- Expression of a RING-HC protein from rice improves resistance to Pseudomonas syringae pv. tomato DC3000 in transgenic Arabidopsis thalianaJournal of Experimental Botany, 2007
- Functions of OsBZR1 and 14-3-3 proteins in brassinosteroid signaling in riceProceedings of the National Academy of Sciences of the United States of America, 2007
- A highly efficient transient protoplast system for analyzing defence gene expression and protein–protein interactions in riceMolecular Plant Pathology, 2006
- The E3 Ubiquitin Ligase BIG BROTHER Controls Arabidopsis Organ Size in a Dosage-Dependent MannerCurrent Biology, 2006
- GS3, a major QTL for grain length and weight and minor QTL for grain width and thickness in rice, encodes a putative transmembrane proteinTheoretical and Applied Genetics, 2006
- Biochemical evidence for ubiquitin ligase activity of the Arabidopsis COP1 interacting protein 8 (CIP8)The Plant Journal, 2002
- A novel gysteine-rich sequence motifCell, 1991