A Tumor Suppressor Enhancer ofPTENin T-cell Development and Leukemia
- 1 January 2021
- journal article
- research article
- Published by American Association for Cancer Research (AACR) in Blood Cancer Discovery
- Vol. 2 (1), 92-109
- https://doi.org/10.1158/2643-3230.bcd-20-0201
Abstract
Long-range oncogenic enhancers play an important role in cancer. Yet, whether similar regulation of tumor suppressor genes is relevant remains unclear. Loss of expression of PTEN is associated with the pathogenesis of various cancers, including T-cell acute lymphoblastic leukemia (T-ALL). Here, we identify a highly conserved distal enhancer (PE) that interacts with the PTEN promoter in multiple hematopoietic populations, including T cells, and acts as a hub of relevant transcription factors in T-ALL. Consistently, loss of PE leads to reduced PTEN levels in T-ALL cells. Moreover, PE-null mice show reduced Pten levels in thymocytes and accelerated development of NOTCH1-induced T-ALL. Furthermore, secondary loss of PE in established leukemias leads to accelerated progression and a gene expression signature driven by Pten loss. Finally, we uncovered recurrent deletions encompassing PE in T-ALL, which are associated with decreased PTEN levels. Altogether, our results identify PE as the first long-range tumor suppressor enhancer directly implicated in cancer. Significance: Here, we identify a PTEN enhancer that is recurrently deleted in patients with T-ALL. Loss of this enhancer leads to reduced PTEN levels in T cells together with accelerated generation and progression of NOTCH1-induced leukemia in vivo. These results uncover long-range regulation of tumor suppressor genes as a relevant mechanism in cancer. This article is highlighted in the In This Issue feature, p. 1Keywords
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Funding Information
- NIH (R00CA197869, R01CA236936)
- American Cancer Society (RSG-19-161-01-TBE)
- NIH (R01CA233662)
- V Foundation (T2019-012)
- Rutgers Cancer Institute NIH (1S10OD012346-01A1)
- NCI (P30CA072720)
- Biomedical Informatics Shared Resource (P30CA072720-5917)
- Flow Cytometry and Cell Sorting Shared Resource (P30CA072720-5921)
- Genome Editing Shared Resource (P30CA072720-5922)
- European Research Council (StG-639784)
- New Jersey Commission on Cancer Research (DCHS20PPC010)
- New Jersey Commission on Cancer Research (DFS18PPC017)
- New Jersey Commission on Cancer Research (DCHS19PPC008)
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