Stbd1 promotes glycogen clustering during endoplasmic reticulum stress and supports survival of mouse myoblasts
Open Access
- 1 October 2020
- journal article
- research article
- Published by The Company of Biologists in Journal of Cell Science
- Vol. 133 (20)
- https://doi.org/10.1242/jcs.244855
Abstract
Imbalances in endoplasmic reticulum (ER) homeostasis provoke a condition known as ER stress and activate the unfolded protein response (UPR) pathway, an evolutionarily conserved cell survival mechanism. Here, we show that mouse myoblasts respond to UPR activation by stimulating glycogenesis and the formation of alpha-amylase-degradable, glycogen-containing ER structures. We demonstrate that the glycogen-binding protein Stbd1 ismarkedly upregulated through the PERK signalling branch of the UPR pathway and is required for the build-up of glycogen structures in response to ER stress activation. In the absence of ER stress, Stbd1 overexpression is sufficient to induce glycogen clustering but does not stimulate glycogenesis. Glycogen structures induced by ER stress are degraded under conditions of glucose restriction through a process that does not depend on autophagosome-lysosome fusion. Furthermore, we provide evidence that failure to induce glycogen clustering during ER stress is associated with enhanced activation of the apoptotic pathway. Our results reveal a so far unknown response ofmousemyoblasts to ERstress and uncover a novel specific function of Stbd1 in this process, which may have physiological implications during myogenic differentiation. This article has an associated First Person interview with the first author of the paper.Funding Information
- Telethon (73159)
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