In order to solve the problem of low CAN bus data transmission rate dur-ing the use of the digital pulse amplitude analysis module in nuclear radiation application scenari-os, a transparent transmission module of RJ45 network and CAN bus was designed. The hardware and software design of the transmission module are introduced in detail, and the actual measure-ment is carried out through the upper computer software developed based on the QT platform, and the energy spectrum data is filtered and displayed. Experiments show that the transparent trans-mission module of the RJ45 network and the CAN bus realizes the bridging of two different commu-nication methods, which ensures the reliability of the data and improves the transmission rate of the digital pulse amplitude analyzer.