A Mixture of D-Amino Acids Enhances the Biocidal Efficacy of CMIT/MIT Against Corrosive Vibrio harveyi Biofilm
Open Access
- 4 September 2020
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Microbiology
Abstract
Biocides are widely used for the mitigation of microbial contamination, especially in the field of the aviation fuel industry. However, the long-term use of biocide has raised the concerns regarding the environmental contamination and microbial drug resistance. In this study, the effect of a mixture of D-amino acids (D-tyrosine and D-methionine) on the enhancement of the bactericidal effect of 5-Chloro-2-Methyl-4-isothiazolin-3-one/2-Methyl-2H-isothiazole-3-one (CMIT/MIT) against corrosive Vibrio harveyi biofilm was evaluated. The results revealed that D-Tyr and D-Met alone can enhance the biocidal efficacy of CMIT/MIT, while the treatment of 5 ppm CMIT/MIT, 1 ppm D-Tyr and 100 ppm D-Met showed the best efficacy comparable to that of 25 ppm CMIT/MIT alone. The triple combination treatment successfully prevented the establishment of the corrosive V. harveyi biofilm and effectively removed the mature V. harveyi biofilm. These conclusions were confirmed by the results of sessile cell counts, images obtained by scanning electron microscope and confocal laser scanning microscope, and the ATP test kit.This publication has 36 references indexed in Scilit:
- The Extracellular Matrix Component Psl Provides Fast-Acting Antibiotic Defense in Pseudomonas aeruginosa BiofilmsPLoS Pathogens, 2013
- Inhibitory Effects of d -Amino Acids on Staphylococcus aureus Biofilm DevelopmentJournal of Bacteriology, 2011
- The biocompatibility and biofilm resistance of implant coatings based on hydrophilic polymer brushes conjugated with antimicrobial peptidesBiomaterials, 2011
- Identification and characterization of microbial contaminants isolated from stored aviation fuels by DNA sequencing and restriction fragment length analysis of a PCR-amplified region of the 16S rRNA geneFuel, 2011
- Microbial Corrosion of Aluminum 2024 Aeronautical Alloy by Hydrocarbon Degrading Bacteria Bacillus cereus ACE4 and Serratia marcescens ACE2Industrial & Engineering Chemistry Research, 2010
- d -Amino Acids Trigger Biofilm DisassemblyScience, 2010
- Combined use of confocal laser scanning microscopy (CLSM) and Raman microscopy (RM): Investigations on EPS – MatrixWater Research, 2009
- Characterization of microbial contamination in United States Air Force aviation fuel tanksJournal of Industrial Microbiology & Biotechnology, 2005
- Heterotrophic plate count measurement in drinking water safety management: Report of an Expert Meeting Geneva, 24–25 April 2002International Journal of Food Microbiology, 2004
- Spatial Variations in Growth Rate within Klebsiella pneumoniae Colonies and BiofilmBiotechnology Progress, 1996