Methylmercury Biosorption Activity by Methylmercury-resistant Lactic Acid Bacteria Isolated From West Sekotong, Indonesia
Open Access
- 1 October 2017
- journal article
- Published by Bogor Agricultural University in HAYATI Journal of Biosciences
- Vol. 24 (4), 182-186
- https://doi.org/10.1016/j.hjb.2017.10.001
Abstract
Methylmercury has been generally known as a toxic heavy metal for both human and environment. Bacterial-based bioremediation of heavy metal is suggested as an ecofriendly and low-cost bioremediation process. There was limited information regarding the role of lactic acid bacteria (LAB) as detoxification agent for methylmercury addressed for human body. West Sekotong, West Lombok, Indonesia, is one of the newly developed artisanal and small-scale gold mining site with high mercury contamination level. This present study was aimed to isolate the human origin methylmercury-resistant LAB and further evaluate their ability to absorb methylmercury. Methylmercury absorption assay was conducted in broth media. The remaining and absorbed methylmercury was measured using the gas chromatography flame ionization detector. A total of 56 methylmercury-resistant LAB isolates were isolated from 37 feces and 19 breast milk samples from 19 volunteers in West Sekotong. Of them, 10 isolates were further selected based on several basic probiotic characteristics and subjected to methylmercury removal assay. The selected isolates showed different methylmercury absorption ability ranged between 17.375 and 51.597 μg/g of wet mass of cell after incubated for 24 hours. Two isolates from feces showing the best removal activity were identified as Enterococcus durans and one isolates from breast milk as Enterococcus faecium based on the sequences of 16s rDNAFunding Information
- Indonesia Toray Science Foundation – 21st Science and Technology Research Grant
This publication has 32 references indexed in Scilit:
- Methylation of Mercury by Bacteria Exposed to Dissolved, Nanoparticulate, and Microparticulate Mercuric SulfidesEnvironmental Science & Technology, 2011
- Organomercurials removal by heterogeneous merB genes harboring bacterial strainsJournal of Bioscience and Bioengineering, 2010
- Chelation in Metal IntoxicationInternational Journal of Environmental Research and Public Health, 2010
- Mercuric resistance genes in gram-positive oral bacteriaFEMS Microbiology Letters, 2004
- Biosorption of toxic metals from aqueous solutions by bacteria strains isolated from metal-polluted soilsProcess Biochemistry, 2004
- Experimental measurements of the reversibility of metal–bacteria adsorption reactionsChemical Geology, 2000
- Resistance of the Normal Human Microflora to Mercury and Antimicrobials After Exposure to Mercury from Dental Amalgam FillingsClinical Infectious Diseases, 1996
- Cloning and comparison of mercury- and organomercurial-resistance determinants from a Pseudomonas stutzeri plasmidGene, 1995
- Comparison of cadmium biosorption by Gram-positive and Gram-negative bacteria from activated sludgeBiotechnology Letters, 1990
- Organomercurial lyase and mercuric ion reductase: nature's mercury detoxification catalystsAccounts of Chemical Research, 1990