Implementation of a High-Repetition-Rate Laser in an AP-SMALDI MSI System for Enhanced Measurement Performance
- 28 December 2020
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Society for Mass Spectrometry
- Vol. 32 (2), 465-472
- https://doi.org/10.1021/jasms.0c00368
Abstract
Matrix-assisted laser desorption/ionization mass spectrometry imaging is a promising tool in the life sciences for obtaining spatial and chemical information from complex biological samples. State-of-the-art setups combine high mass resolution and high mass accuracy with high lateral resolution, offering untargeted insights into biochemical processes on the single-cell length scale. Despite recent technological breakthroughs, the sensitivity and acquisition speed of many setups are often in competition with achievable pixel resolutions below 25 μm. New measurement modes were developed by implementing a high-repetition-rate laser into an AP-SMALDI10 ion source, coupled to an orbital trapping mass spectrometer. These new MSI modes allow for a modular use of the new setup. We demonstrate that the system allows single cell features to be visualized in mouse brain tissue sections at a pixel resolution of 5 μm and an imaging speed of 18 pixels/s. Furthermore, the analytical sensitivity was improved in another measurement mode by applying multiple pulses of a highly focused laser beam over larger square pixels ≥25 μm edge length, increasing ion signal intensities up to 20-fold on tissue and decreasing the limit of detection by 1 order of magnitude.Keywords
Funding Information
- Deutsche Forschungsgemeinschaft (He8521/1-1, INST 162/500-1 FUGG, Sp314/13-1)
- Verband der Chemischen Industrie
- State of Hesse
This publication has 28 references indexed in Scilit:
- Mass Spectrometry Imaging for Dissecting Steroid Intracrinology within Target TissuesAnalytical Chemistry, 2013
- Mirion—A Software Package for Automatic Processing of Mass Spectrometric ImagesJournal of the American Society for Mass Spectrometry, 2013
- Localizing Genes to Cerebellar Layers by Classifying ISH ImagesPLoS Computational Biology, 2012
- High-Speed MALDI-TOF Imaging Mass Spectrometry: Rapid Ion Image Acquisition and Considerations for Next Generation InstrumentationJournal of the American Society for Mass Spectrometry, 2011
- MALDI produced ions inspected with a linear ion trap-orbitrap hybrid mass analyzerJournal of the American Society for Mass Spectrometry, 2009
- A high‐resolution scanning microprobe matrix‐assisted laser desorption/ionization ion source for imaging analysis on an ion trap/Fourier transform ion cyclotron resonance mass spectrometerRapid Communications in Mass Spectrometry, 2008
- The effect of laser profile, fluence, and spot size on sensitivity in orthogonal‐injection matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometryRapid Communications in Mass Spectrometry, 2008
- LMSD: LIPID MAPS structure databaseNucleic Acids Research, 2006
- The Orbitrap: a new mass spectrometerJournal of Mass Spectrometry, 2005
- Fourier transform ion cyclotron resonance spectroscopyChemical Physics Letters, 1974