Study on Separation and Purification the Secondary Metabolites of Oudemansiella Mucida
Open Access
- 1 March 2020
- journal article
- research article
- Published by IOP Publishing in IOP Conference Series: Materials Science and Engineering
- Vol. 782 (2)
- https://doi.org/10.1088/1757-899x/782/2/022008
Abstract
Oscillation culture was carried out on Oudemansiella mucida. The mycelia were extracted with ethyl acetate, separated by silica column chromatography and Sephadex LH-20 dextran gel chromatography. Though determination of chemical and chemical properties of chemical constituents, characterization by organic spectra (MS, 1H NMR, 13C NMR, and DEPT), and comparison with standard compounds, the three secondary metabolites from mycelia were finally identified to be ergosta-4, 6, 8(14), 22-tetraen-3-one; 5α, 8α-epidioxy-(22E, 24R)-ergosta-6, 22-dien-3β-ol, Hexahydropyrrolo [1, 2-α] pyrazine-1,4-dione.This publication has 5 references indexed in Scilit:
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