Perception of the pathogen‐induced peptide RGF7 by the receptor‐like kinases RGI4 and RGI5 triggers innate immunity in Arabidopsis thaliana
- 17 January 2021
- journal article
- research article
- Published by Wiley in New Phytologist
- Vol. 230 (3), 1110-1125
- https://doi.org/10.1111/nph.17197
Abstract
Signaling peptides play crucial roles in plant growth, development and defense. We report here that the Arabidopsis thaliana secreted peptide, ROOT MERISTEM GROWTH FACTOR 7 (RGF7), functions as an endogenous elicitor to trigger plant immunity. Expression of the RGF7 precursor‐encoding gene (preRGF7) is highly induced in Arabidopsis leaves upon infection by the bacterial pathogen Pseudomonas syringae. The pathogen‐responsive preRGF7 expression is regulated by the transcription factor WRKY33 and its upstream protein kinases MPK3/MPK6 and CPK5/CPK6. In the absence of pathogen attack, chemically induced expression of preRGF7 in transgenic Arabidopsis plants was sufficient to trigger immune responses. Pre‐induction of preRGF7 expression in transgenic Arabidopsis also led to enhanced immune responses and increased resistance to P. syringae infection. Biochemical and genetic analyses demonstrated that RGF7 is perceived by the leaf‐expressed RGF1 INSENSITIVE (RGI) family receptors RGI4 and RGI5. The SOMATIC EMBRYOGENESIS RECEPTOR KINASES (SERKs) BAK1 and SERK4 are also involved in RGF7 perception via forming RGF7‐induced receptor‐complexes with RGI4 and RGI5. These results indicate that the pathogen‐induced RGF7 peptide, perceived by the RGI4/RGI5‐BAK1/SERK4 receptor complexes, acts as a new damage‐associated molecular pattern (DAMP) and plays a significant role in Arabidopsis immunity.Keywords
Funding Information
- National Natural Science Foundation of China (31800215, 31970282)
This publication has 46 references indexed in Scilit:
- Induced Genome-Wide Binding of Three Arabidopsis WRKY Transcription Factors during Early MAMP-Triggered ImmunityTHE PLANT CELL ONLINE, 2016
- Regulation of pattern recognition receptor signalling in plantsNature Reviews Immunology, 2016
- Multilayered Regulation of Ethylene Induction Plays a Positive Role in Arabidopsis Resistance against Pseudomonas syringaePlant Physiology, 2015
- Effector-Triggered Immunity: From Pathogen Perception to Robust DefenseAnnual Review of Plant Biology, 2015
- Transcriptional and Functional Classification of the GOLVEN/ROOT GROWTH FACTOR/CLE-Like Signaling Peptides Reveals Their Role in Lateral Root and Hair FormationPlant Physiology, 2012
- A Renaissance of Elicitors: Perception of Microbe-Associated Molecular Patterns and Danger Signals by Pattern-Recognition ReceptorsAnnual Review of Plant Biology, 2009
- Tyrosine-sulfated glycopeptide involved in cellular proliferation and expansion in ArabidopsisProceedings of the National Academy of Sciences of the United States of America, 2007
- A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defenceNature, 2007
- FLS2: An LRR Receptor–like Kinase Involved in the Perception of the Bacterial Elicitor Flagellin in ArabidopsisMolecular Cell, 2000
- Floral dip: a simplified method forAgrobacterium‐mediated transformation ofArabidopsis thalianaThe Plant Journal, 1998