Background and aim: A one-step prostate-specific antigen (PSA) test was studied in a large population to improve the convenience and lower the cost of prostate cancer (PC) screening. The PSA rapid test kit aids in the diagnosis of PC by detecting human PSA in serum or plasma at or above a threshold level of 4 ng/mL. The aim of this study is to screen PC using a Quick One-Step PSA Test. Materials and Methods: Between July 2020 and August 2021, at National institute of urology and nephrology, Cairo, Egypt, Male attendees were offered PC screening utilizing the PSA quick test. A total of 305 men's blood samples were tested. The test was interpreted by two independent observers. Following that, the remaining serum samples were examined using a standard quantitative technique. Results: Of 305 participants, 118 had a PSA value of less than 4 ng/mL, and 55 presented a PSA value higher than 4 ng/mL. A total of 63 participants had a PSA value between 4 and 10 ng/mL. A total of 12 participants presented a PSA value > 10 ng/mL; 10 was judged as positive. The intensity of the color reaction was implying a strong positive correlation between the two methods (correlation test: P< 0.0001). Conclusion: The one-step test is a good preliminary screening method from which positive results may be quantified to provide a semi-quantitative estimate of the degree of PSA rise. The test is inexpensive, easy to do, and provides results in a timely manner.