The Allelic Diversity of the Gibberellin Signaling Pathway Genes in Aegilops tauschii Coss
Open Access
- 2 December 2020
- Vol. 9 (12), 1696
- https://doi.org/10.3390/plants9121696
Abstract
Gibberellin-insensitive reduced height genes are widely spread in modern wheat varieties, making them resistant to lodging under conditions of intensive farming. However, the limited diversity of these genes present in wheat germplasm can limit the adaptability of newly created cultivars to the changing climate. The diversity of the gibberellin signaling pathway genes involved in plant height control—Reduced height 1 (Rht-D1), Gibberellin-insensitive dwarf 1 (Gid1-D) and Gibberellin-insensitive dwarf 2 (Gid2-D)—was studied in the diploid wild goatgrass Aegilops tauschii Coss., one of the ancestral species of the bread wheat (Triticum aestivum L.) and the donor of its D subgenome, using high-throughput sequencing. The examination of 24 Ae. tauschii accessions of different geographical origins revealed a large number of new alleles (haplotypes) not found in bread wheat varieties. Some of the detected polymorphisms lead to changes in the amino acid sequence of proteins. Four isoforms (amino acid sequence variants) were found for the RHT-D1 protein, and two isoforms—for the GID1 and GID2 proteins, each. An analysis of the co-occurrence frequencies of various isoforms of the three proteins showed that their combinations were not random in Ae. tauschii, which may indicate the functional significance of their differences. New alleles of the Rht-D1, Gid1-D, and Gid2-D genes are promising for introgression into bread wheat and studying their effect on plant height and adaptability.Keywords
This publication has 47 references indexed in Scilit:
- Predicting the Functional Effect of Amino Acid Substitutions and IndelsPLOS ONE, 2012
- Reduced height genes and their importance in winter wheat cultivars grown in southern RussiaEuphytica, 2012
- Mathematical Modelling Long-Term Effects of Replacing Prevnar7 with Prevnar13 on Invasive Pneumococcal Diseases in England and WalesPLOS ONE, 2012
- Primer-BLAST: A tool to design target-specific primers for polymerase chain reactionBMC Bioinformatics, 2012
- SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell SequencingJournal of Computational Biology, 2012
- Molecular Characterization ofRht-1Dwarfing Genes in Hexaploid WheatPlant Physiology, 2011
- Dominant and Pleiotropic Effects of aGAIGene in Wheat Results from a Lack of Interaction between DELLA and GID1Plant Physiology, 2011
- GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellinNature, 2005
- MUSCLE: multiple sequence alignment with high accuracy and high throughputNucleic Acids Research, 2004
- Physiological and Agronomic Consequences of Rht Genes in WheatJournal of Crop Production, 1997