Editorial: New Insights Into Thymic Functions During Stress, Aging, and in Disease Settings

Abstract

Editorial on the Research Topic New Insights Into Thymic Functions During Stress, Aging, and in Disease Settings The thymus is a bi-lobed lymphoid organ localized above the heart whose primary function is to foster the development of the T cells of the adaptive immune system. T cells are a critical component of the cellular immune system, helping B cells produce antibodies, releasing cytokines to orchestrate effective immune responses, and killing infected cells and/or neoplasm/tumor cells. The recognition of an infected cell or tumor cell, and the ability to support B cell secretion of antibodies requires T cells express a cell surface receptor, termed the T cell receptor (TCR). This receptor selectively binds to peptides complexed to major histocompatibility complex antigens (MHC class I and II). MHC class I molecules are present on all nucleated cells in the body, while MHC class II is restricted to antigen presenting cells. In the thymus, thymocytes rearrange DNA loci comprising the genes encoding the TCR subunits. Using a process termed VDJ recombination, each thymocyte expresses a unique TCR with exclusive recognition specificities. However, only those thymocytes expressing TCRs that can engage self-peptides/self MHC complexes present on thymic epithelial cells (TECs) are “selected” to form the peripheral T cell repertoire. This involves processes coined positive and negative selection, with the ensuing repertoire of T cells capable of recognizing pathogen- or distinct tumor- derived peptides presented on self-MHC molecules without overt reactivity to self-peptides. The critical role of the thymus in this process is best exemplified with infants born with mutations in genes required for the formation of the thymus (22q11.2 deletion syndrome or DiGeorge). No thymus results in no T cells, leading to a severe combined immunodeficiency. A second excellent example pertains to the spontaneously arising nude mouse, which lacks a thymus and hair due to mutations in the Forkhead Box N1 transcription factor. This transcription factor supports the development of TECs, the controllers of T cell fate, and defective TECs means no T cells. Yet, as late as 1960, the thymus was a scientific conundrum. Dramatic variations in its size in humans and diverse species had been documented for over two millennia. Severe clinical repercussions based on these size differences ensued due to a complete lack of knowledge about its functions, which was not revealed until demonstrated in pivotal experiments performed by Jacques Miller using thymectomy and skin grafting. These experiments established the thymus as the site of T cell development. For this, Miller was awarded the Lasker Prize in Biomedicine in 2019 (1). Between the 1890s–1950s, an enlarged thymic tissue noted in newborns and infants was proposed causal to asthma and crib death (thymicolymphaticus) (2). This unsubstantiated pathological connection led to tens of thousands of infants receiving “therapeutic” radiation treatments of 0.2–2 Gy to wipe out the thymus (3). Such an ill-conceived therapy resulted in >10,000 children developing thyroid cancers. A subsequent and less damaging treatment to reduce thymus size involved steroid injections. While not as severely marred as with the high radiation doses, the thymus could again be rendered hypoplastic. These therapies have revealed the extreme stress sensitivity of the thymus, but also its remarkable capacity for repair. In a normal setting, thymic function reaches its natural peak during the neonatal and pre-adolescent period, thereafter the thymus begins to decrease in size and activity. This process, termed thymic involution, results in significant loss in the production of T cells although some T cell development continues throughout adult life. This dynamic process, both with chronic age-related declines and acute atrophy caused by steroids or cytoreductive chemotherapy, can have profound negative impacts in the efficacy of adaptive immunity. Today, understanding how to maintain the function of the thymus through-out life, particularly after damage, is the driving goal of researchers. In the series of articles under the title “New Insights into thymic functions during stress, aging, and in disease settings” in this issue of Frontiers in Immunology, novel insights and findings by leading scientists in the field of thymus biology are presented. The articles include two reviews outlining recent discoveries into the mechanisms required for the formation and specification of the thymus within the 3rd pharyngeal pouch during embryogenesis (Bhalla et al.; Giardino et al.). The contributions of key transcription factors such as T Box Transcription Factor 1 (TBX1) and FOXN1 in the patterning of the pharyngeal apparatus and the subsequent demarcation of the thymus anlage are discussed in conjunction with the differentiation and expansion of thymic epithelial cells. The formation of the two different populations of epithelial cells, cortical and medullary TECs and their selective roles in supporting and educating thymocytes to form the repertoire of T cells, which is unique to each individual, is described, revealing new subsets of these cells in the process (Alawam et al.). As is well-recognized currently, in addition to aging, the thymus undergoes a transient and sometimes massive reduction in overall size and cellularity following infections, corticosteroid production through the hypothalamus-pituitary-adrenal axis or via injections, and chemotherapy treatments. The impact on thymocytes and stromal cell populations are presented herein (Gaballa et al.; Kinsella and Dudakov). Such thymic hypoplasia is widespread in the population, and the different conditions leading to hypoplasia/aplasia of the thymus are presented in a combination of research and review articles in this series (Cowan et...