The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes
Open Access
- 7 December 2020
- Vol. 9 (12), 2641
- https://doi.org/10.3390/cells9122641
Abstract
Mechanotransduction is the ability of cells to translate mechanical stimuli into biochemical signals that can ultimately influence gene expression, cell morphology and cell fate. Tenocytes are responsible for tendon mechanical adaptation converting mechanical stimuli imposed during mechanical loading, thus affecting extracellular matrix homeostasis. Since we previously demonstrated that MD-Tissue, an injectable collagen-based medical compound containing swine-derived collagen as the main component, is able to affect tenocyte properties, the aim of this study was to analyze whether the effects triggered by MD-Tissue were based on mechanotransduction-related mechanisms. For this purpose, MD-Tissue was used to coat Petri dishes and cytochalasin B was used to deprive tenocytes of mechanical stimulation mediated by the actin cytoskeleton. Cell morphology, migration, collagen turnover pathways and the expression of key mechanosensors were analyzed by morphological and molecular methods. Our findings confirm that MD-Tissue affects collagen turnover pathways and favors cell migration and show that the MD-Tissue-induced effect represents a mechanical input involving the mechanotransduction machinery. Overall, MD-Tissue, acting as a mechanical scaffold, could represent an effective medical device for a novel therapeutic, regenerative and rehabilitative approach to favor tendon healing in tendinopathies.This publication has 57 references indexed in Scilit:
- Tendon Biomechanics and Mechanobiology—A Minireview of Basic Concepts and Recent AdvancementsJournal of Hand Therapy, 2012
- In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitroNature Protocols, 2007
- Tissue Cells Feel and Respond to the Stiffness of Their SubstrateScience, 2005
- Cyclic tensile strain upregulates collagen synthesis in isolated tendon fasciclesBiochemical and Biophysical Research Communications, 2005
- Tissue-specific expression and regulation of the alternatively-spliced forms of lysyl hydroxylase 2 (LH2) in human kidney cells and skin fibroblastsMatrix Biology, 2005
- Effect of Amplitude and Frequency of Cyclic Tensile Strain on the Inhibition of MMP-1 mRNA Expression in Tendon Cells: An In Vitro StudyConnective Tissue Research, 2003
- Mechanical load stimulates expression of novel genesin vivoandin vitroin avian flexor tendon cellsOsteoarthritis and Cartilage, 1999
- Effect of stress deprivation and cyclic tensile loading on the material and morphologic properties of canine flexor digitorum profundus tendon: An in vitro studyJournal of Orthopaedic Research, 1995
- Regulation of Matrix Metalloproteinase ActivityaAnnals of the New York Academy of Sciences, 1994
- Glycosaminoglycans of human rotator cuff tendons: changes with age and in chronic rotator cuff tendinitis.Annals Of The Rheumatic Diseases, 1994