miR-346-3p promotes osteoclastogenesis via inhibiting TRAF3 gene
- 1 August 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in In Vitro Cellular & Developmental Biology – Animal
- Vol. 56 (7), 533-542
- https://doi.org/10.1007/s11626-020-00479-w
Abstract
MicroRNAs (miRNAs) modulate gene expression and regulate many physiological and pathological conditions. However, their modulation and effect in osteoclastogenesis remain unknown. In this study, we investigated the role of miR-346-3p in regulating the osteoclast differentiation from RAW264.7 cells. We used the miRNA microarray assay, miR-346-3p mimic transfection, tartrate resistant acid phosphatase (TRAP) staining, bone resorption assay, qRT-PCR, and western blot. Our results showed that the expression of miR-346-3p was significantly upregulated during osteoclast differentiation. Further, by transfecting cells with miR-346-3p mimic, we observed an increased number of TRAP-positive multinucleated cells, increased pit area caused by bone resorption, and enhanced expression of osteoclast-specific genes and proteins. Conversely, miR-346-3p inhibition attenuated the osteoclast differentiation and function. Software-mediated prediction and validation using luciferase reporter assay showed that TRAF3, a negative regulator of osteoclast differentiation, was inhibited by miR-346-3p overexpression. Our results showed that miR-346-3p directly targeted TRAF3 mRNA via binding to its 3 '-UTR and inhibited the expression of TRAF3 protein. Taken together, our results revealed that miR-346-3p promotes the regulation of osteoclastogenesis by suppressing the TRAF3 gene. In conclusion, miR-346-3p could be a novel therapeutic target for bone loss-related pathogenesis.Funding Information
- National Natural Science Foundation of China (31700854)
- Natural Science Foundation of Anhui Province (1908085MH276)
- Key Program of Anhui Educational Committee (KJ2018A1011)
- Key Program of Anhui Educational Committee (KJ2019A0392)
- Scientific Research Foundation of Bengbu Medical College (BYKY17118, BYKY1848ZD)
- Scientific Research Foundation of Bengbu Medical College (BYKY18108)
- Translational Medicine Key Projects of Bengbu Medical College (BYTM2019006)
- Translational Medicine Key Projects of Bengbu Medical College (BYTM2019012)
- Scientific Research Innovation Team of Bengbu Medical (BYKC201910)
- Postgraduate Research Innovation Project of Bengbu Medical College (Byycx1827, Byycx1954)
- Postgraduate Research Innovation Project of Bengbu Medical College (Byycx1956)
- Postgraduate Research Innovation Project of Bengbu Medical College (Byycz1929)
- National Training Programs of Innovation and Entrepreneurship for Undergraduate (201810367013)
- National Training Programs of Innovation and Entrepreneurship for Undergraduate (201810367028)
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