Preliminary Assessment of the Mucosal Toxicity of Tea Tree (Melaleuca alternifolia) and Rosemary (Rosmarinus officinalis) Essential Oils on Novel Porcine Uterus Models
Open Access
- 9 May 2020
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 21 (9), 3350
- https://doi.org/10.3390/ijms21093350
Abstract
Antimicrobial resistance, an ever-growing global crisis, is strongly linked to the swine production industry. In previous studies, Melaleuca alternifolia and Rosmarinus officinalis essential oils have been evaluated for toxicity on porcine spermatozoa and for antimicrobial capabilities in artificial insemination doses, with the future perspective of their use as antibiotic alternatives. The aim of the present research was to develop and validate in vitro and ex vivo models of porcine uterine mucosa for the evaluation of mucosal toxicity of essential oils. The in vitro model assessed the toxicity of a wider range of concentrations of both essential oils (from 0.2 to 500 mg/mL) on sections of uterine tissue, while the ex vivo model was achieved by filling the uterine horns. The damage induced by the oils was assessed by Evans Blue (EB) permeability assay and histologically. The expression of ZO-1, a protein involved in the composition of tight junctions, was assessed through immunohistochemical and immunofluorescence analysis. The results showed that low concentrations (0.2–0.4 mg/mL) of both essential oils, already identified as non-spermicidal but still antimicrobial, did not alter the structure and permeability of the swine uterine mucosa. Overall, these findings strengthen the hypothesis of a safe use of essential oils in inseminating doses of boar to replace antibiotics.This publication has 53 references indexed in Scilit:
- Effect of Essential Oils on Pathogenic BacteriaPharmaceuticals, 2013
- The perfused swine uterus model: long-term perfusionReproductive Biology and Endocrinology, 2012
- Barrier effect of Esoxx® on esophageal mucosal damage: experimental study on ex-vivo swine modelClinical and Experimental Gastroenterology, 2012
- Terpinen-4-ol Induces Apoptosis in Human Nonsmall Cell Lung Cancer In Vitro and In VivoEvidence-Based Complementary and Alternative Medicine, 2012
- Reproductive performance of sows submitted to intrauterine inseminationRevista Brasileira de Zootecnia, 2009
- Evaluation of swine fertilisation medium (SFM) efficiency in preserving spermatozoa quality during long-term storage in comparison to four commercial swine extendersAnimal, 2009
- Porcine Vagina Ex Vivo as a Model for Studying Permeability and Pathogenesis in MucosaJournal of Pharmaceutical Sciences, 2008
- Melaleuca alternifolia(Tea Tree) Oil: a Review of Antimicrobial and Other Medicinal PropertiesClinical Microbiology Reviews, 2006
- Trophoblastic Interferons: Do they Modulate Uterine Cellular Markers at the Time of Conceptus Attachment in the Pig?Placenta, 2003