Label-free histomorphometry of peripheral nerve by stimulated Raman spectroscopy
- 1 July 2020
- journal article
- research article
- Published by Wiley in Muscle & Nerve
- Vol. 62 (1), 137-142
- https://doi.org/10.1002/mus.26895
Abstract
Background Conventional processing of nerve for histomorphometry is resource-intensive, precluding use in intraoperative assessment of nerve quality during nerve transfer procedures. Stimulated Raman scattering (SRS) microscopy is a label-free technique that enables rapid and high-resolution histology. Methods Segments of healthy murine sciatic nerve, healthy human obturator nerve, and human cross-facial nerve autografts were imaged on a custom SRS microscope. Myelinated axon quantification was performed through segmentation using a random forest machine learning algorithm in commercial software. Results High contrast, high-resolution imaging of nerve morphology was obtained with SRS imaging. Automated myelinated axon quantification from cross-sections of healthy human nerve imaged using SRS was achieved. Conclusions Herein, we demonstrate the use of a label-free technique for rapid imaging of murine and human peripheral nerve cryosections. We illustrate the potential of this technique to inform intraoperative decision-making through rapid automated quantification of myelinated axons using a machine learning algorithm.Funding Information
- National Institute of Neurological Disorders and Stroke (R01NS071067)
- Harvard University
- National Institutes of Health
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