The smfBox is an open-source platform for single-molecule FRET
Open Access
- 6 November 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Communications
- Vol. 11 (1), 1-6
- https://doi.org/10.1038/s41467-020-19468-4
Abstract
Single-molecule Förster Resonance Energy Transfer (smFRET) is a powerful technique capable of resolving both relative and absolute distances within and between structurally dynamic biomolecules. High instrument costs, and a lack of open-source hardware and acquisition software have limited smFRET’s broad application by non-specialists. Here, we present the smfBox, a cost-effective confocal smFRET platform, providing detailed build instructions, open-source acquisition software, and full validation, thereby democratising smFRET for the wider scientific community.Keywords
Funding Information
- RCUK | Engineering and Physical Sciences Research Council
- RCUK | STFC | Central Laser Facility, Science and Technology Facilities Council
- EC | Erasmus+
- RCUK | Biotechnology and Biological Sciences Research Council (BB/T008032/1)
- Royal Society (RGS\R2\180405)
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