Production and Characterization of Biosurfactant byNocardiaSpecies Isolated Form Soil Samples in Tehran
- 1 January 2021
- journal article
- research article
- Published by Walter de Gruyter GmbH in Tenside Surfactants Detergents
- Vol. 58 (1), 74-80
- https://doi.org/10.1515/tsd-2020-2263
Abstract
In this work, we report the Nocardia species were initially screened and then tested for their ability to produce biosurfactant. The biosurfactant production from the Nocardia species was determined by qualitative standard methods. The selected bacterial strain had better oil spreading and emulsifying activity and surface tension reduction. The biosurfactant producer strain was identified by 16S rRNA gene sequencing method. Using Fourier transform infrared spectroscopy and comparative analysis, the biosurfactant structure could be detected. Oil spreading tests and blue agar plate tests confirmed the presence of biosurfactant and extracellular anionic glycolipids. The E24% assay using olive oil showed strong emulsifying characteristic of extracted biosurfactant with a 100%. The stability of the biosurfactant produced in different conditions was significant. Nocardia coubleae was identified by biochemical methods and molecular methods.Keywords
This publication has 21 references indexed in Scilit:
- Biosurfactants: Promising Molecules for Petroleum Biotechnology AdvancesFrontiers in Microbiology, 2016
- Biosurfactant Producing Microbes and their Potential Applications: A ReviewCritical Reviews in Environmental Science and Technology, 2014
- Biosurfactant production from marine bacteria associated with sponge Callyspongia diffusa3 Biotech, 2014
- Characterization and stability of biosurfactant produced byBacillus mojavensisA21 and its application in enhancing solubility of hydrocarbonJournal of Chemical Technology & Biotechnology, 2013
- Production and stability studies of the biosurfactant isolated from marine Nocardiopsis sp. B4Desalination, 2012
- Production of biosurfactant by Nocardia otitidiscaviarum and its role in biodegradation of crude oilInternational Journal of Environmental Science and Technology, 2011
- Isolation, characterization and biological evaluation of bioactive metabolites from Nocardia levis MK-VL_113Microbiological Research, 2010
- Screening Concepts for the Isolation of Biosurfactant Producing MicroorganismsAdvances in experimental medicine and biology, 2010
- Synthesis of Biosurfactants and Their Advantages to Microorganisms and MankindAdvances in experimental medicine and biology, 2010
- Production and characterization of lipopeptide biosurfactant by a sponge-associated marine actinomycetes Nocardiopsis alba MSA10Bioprocess and Biosystems Engineering, 2009