Smarca5-mediated epigenetic programming facilitates fetal HSPC development in vertebrates
Open Access
- 14 January 2021
- journal article
- research article
- Published by American Society of Hematology in Blood
- Vol. 137 (2), 190-202
- https://doi.org/10.1182/blood.2020005219
Abstract
Nascent hematopoietic stem and progenitor cells (HSPCs) acquire definitive hematopoietic characteristics only when they develop into fetal HSPCs; however, the mechanisms underlying fetal HSPC development are poorly understood. Here, we profiled the chromatin accessibility and transcriptional features of zebrafish nascent and fetal HSPCs using ATAC-seq and RNA-seq and revealed dynamic changes during HSPC transition. Functional assays demonstrated that chromatin remodeler-mediated epigenetic programming facilitates fetal HSPC development in vertebrates. Systematical screening of chromatin remodeler-related genes identified that smarca5 is responsible for the maintenance of chromatin accessibility at promoters of hematopoiesis-related genes in fetal HSPCs. Mechanistically, Smarca5 interacts with nucleolin to promote chromatin remodeling, thereby facilitating genomic binding of transcription factors to regulate expression of hematopoietic regulators such as bcl11ab. Our results unravel a new role of epigenetic regulation and reveal that Smarca5-mediated epigenetic programming is responsible for fetal HSPC development, which will provide new insights into the generation of functional HSPCs both in vivo and in vitro.This publication has 57 references indexed in Scilit:
- The Transcriptional Landscape of Hematopoietic Stem Cell OntogenyCell Stem Cell, 2012
- Up-regulation of Cavβ3 Subunit in Primary Sensory Neurons Increases Voltage-activated Ca2+ Channel Activity and Nociceptive Input in Neuropathic PainOnline Journal of Public Health Informatics, 2012
- The identification and characterization of zebrafish hematopoietic stem cellsBlood, 2011
- Haematopoietic stem cells derive directly from aortic endothelium during developmentNature, 2010
- CEAS: cis-regulatory element annotation systemBioinformatics, 2009
- Hematopoiesis: An Evolving Paradigm for Stem Cell BiologyCell, 2008
- An Essential Switch in Subunit Composition of a Chromatin Remodeling Complex during Neural DevelopmentNeuron, 2007
- The zebrafish lysozyme C promoter drives myeloid-specific expression in transgenic fishBMC Developmental Biology, 2007
- Nucleolin Regulates Gene Expression in CD34-positive Hematopoietic CellsOnline Journal of Public Health Informatics, 2007
- Nucleolin is a histone chaperone with FACT-like activity and assists remodeling of nucleosomesThe EMBO Journal, 2006