DARKFIELD MICROSCOPY (DFM) IN THE EVALUATION OF EXTRACELLULAR VESICLES

Abstract

Extracellular vesicles (EVs) are particles ranging from 30 to 5000 nm, considered mediators of intercellular communication and biomarkers of distinct types of diseases. EVs are released by most cells, including tumor cells. Therefore, research to better understand its role in the development and progression of neoplasms has been increasing every year. For such, these studies require high-tech equipment, available in highly complex research laboratories. This project sought to standardize dark-field microscopy (DFM) as a simple, effective, low-cost method to assist in research with EVs. Three samples were acquired, each one containing extracellular vesicles properly classified by size (400, 100, and 50 nm). Each one of them was analyzed by using the DFM to determine resolution capability and maximum magnification.