Signal amplification strategies for clinical biomarker quantification using elemental mass spectrometry
- 5 March 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Analytical and Bioanalytical Chemistry
- Vol. 414 (1), 53-62
- https://doi.org/10.1007/s00216-021-03251-5
Abstract
The current trends in modern medicine towards early diagnosis, or even prognosis, of different diseases have brought about the need for the corresponding biomarker detection at ever lower levels in really complex matrices. To do so, it is necessary to use proper extremely sensitive detection techniques such as elemental mass spectrometry. However, target labelling with metals for subsequent sensitive ICP-MS detection falls short nowadays even if resorting to inorganic nanoparticles containing a high number of detectable elements. Thus, new amplification strategies are being proposed to face this analytical challenge that will be critically discussed in this paper. Fundamentals of different novel strategies developed to achieve signal amplification and sensitive elemental mass spectrometry detection are here discussed. Some representative examples of relevant clinical applications are highlighted, along with future prospects and challenges. Graphical abstractKeywords
Funding Information
- Spanish Ministry of Economy and Competitiveness (PID2019- 109698GB-I00)
- Government of Principado de Asturias (FC-GRUPIN-IDI/2018/000166)
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