Abstract
The loss on ignition (LOI) of fatty acids (FAs; including carbon numbers of C8–C24) in vegetal and animal organic reference samples at 200–500°C was examined. The total FAs in the samples preferentially disappeared through LOI operation compared to the other compounds. The average carbon numbers in total FAs did not change substantially from their original values due to LOI, regardless of the temperature. However, the unsaturation ratios in each FA species fluctuated throughout the operation.