Mediation of Potato–Potato Cyst Nematode, G. rostochiensis Interaction by Specific Root Exudate Compounds
Open Access
- 10 June 2020
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Plant Science
- Vol. 11, 649
- https://doi.org/10.3389/fpls.2020.00649
Abstract
Potato (Solanum tuberosum) is a widely consumed staple food crop worldwide whose production is threatened by potato cyst nematodes (PCN). To infect a host, PCN eggs first need to be stimulated to hatch by chemical components in the host root exudates, yet it remains unknown how most root exudate components influence PCN behavior. Here, we evaluated the influence of eight compounds identified by LC-QqQ-MS in the root exudate of potato on the hatching response of the PCN, Globodera rostochiensis at varying doses. The eight compounds included the amino acids tyrosine, tryptophan and phenylalanine; phytohormones zeatin and methyl dihydrojasmonate; steroidal glycoalkaloids α-solanine and α-chaconine and the steroidal alkaloid solanidine. We additionally tested two other Solanaceae steroidal alkaloids, solasodine and tomatidine, previously identified in the root exudates of tomato, an alternative host for PCN. In dose-response assays with the individual compounds, the known PCN hatching factors α-chaconine and α-solanine stimulated the highest number of eggs to hatch, ∼47 and ∼42%, respectively, whereas the steroidal alkaloids (aglycones), solanidine and solasodine and potato root exudate (PRE) were intermediate, 28% each and 21%, respectively, with tomatidine eliciting the lowest hatching response 13%. However, ∼60% of the hatched juveniles failed to emerge from the cyst, which was compound- and concentration-dependent. The amino acids, phytohormones and the negative control (1% DMSO in water), however, were generally non-stimulatory. The use of steroidal glycoalkaloids and their aglycones in the suicidal hatching of PCN offers promise as an environmentally sustainable approach to manage this pest.Keywords
Funding Information
- North Carolina State University
- Bill and Melinda Gates Foundation
- Government of the Republic of Kenya
- Direktion für Entwicklung und Zusammenarbeit
- Styrelsen för Internationellt Utvecklingssamarbete
- Department for International Development, UK Government
This publication has 53 references indexed in Scilit:
- Methods of collection of plant root exudates in relation to plant metabolism and purpose: A reviewJournal of Plant Nutrition and Soil Science, 2013
- Abamectin and Azadirachtin as Eco-friendly Promising Biorational Tools in Integrated Nematodes Management ProgramsJournal of Plant Pathology & Microbiology, 2013
- The Composition of Root Exudates from Two Different Resistant Peanut Cultivars and Their Effects on the Growth of Soil-Borne PathogenInternational Journal of Biological Sciences, 2013
- Antifungal Activity and Fungal Metabolism of Steroidal Glycosides of Easter Lily (Lilium longiflorum Thunb.) by the Plant Pathogenic Fungus, Botrytis cinereaJournal of Agricultural and Food Chemistry, 2011
- Root exudation of sugars, amino acids, and organic acids by maize as affected by nitrogen, phosphorus, potassium, and iron deficiencyJournal of Plant Nutrition and Soil Science, 2010
- Streptomyces scabies87-22 Possesses a Functional TomatinaseJournal of Bacteriology, 2008
- LC-MS Analysis of Solanidane Glycoalkaloid Diversity among Tubers of Four Wild Potato Species and Three Cultivars (Solanum tuberosum)Journal of Agricultural and Food Chemistry, 2008
- ROOT EXUDATES AND MICROORGANISMSPublished by Elsevier BV ,2005
- Detection of hatching inhibitors and hatching factor stimulants for golden potato cyst nematode, Globodera rostochiensis, in potato root leachateAnnals of Applied Biology, 1998
- Amino acids in root exudates of cottonPlant and Soil, 1962