Protective effects of methanolic leaf extracts of Monanthotaxis caffra against aflatoxin B1-induced hepatotoxicity in rats
Open Access
- 10 January 2022
- journal article
- research article
- Published by AOSIS in Onderstepoort Journal of Veterinary Research
- Vol. 89 (1), 6-e6
- https://doi.org/10.4102/ojvr.v89i1.1968
Abstract
Aflatoxins are potent hepatotoxic and carcinogenic secondary metabolites produced by toxigenic fungi. The present study investigated the protective effect of methanolic leaf extracts of Monanthotaxis caffra (MLEMC) against aflatoxin B1-induced toxicity in male Sprague-Dawley rats. The rats were randomly divided into 6 groups of 8 animals each. Five groups were administered orally for seven days with three different concentrations of MLEMC (100 mg/kg, 200 mg/kg and 300 mg/kg), curcumin (10 mg/kg) or vehicle (25% propylene glycol). The following day, these groups were administered 1 mg/kg b.w. of aflatoxin B1 (AFB1). The experiment was terminated three days after administration of AFB1. Group 6 represented untreated healthy control. Serum aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, creatinine and liver histopathology were evaluated. Methanolic leaf extracts of M. caffra decreased the levels of aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase and creatinine in the sera of rats as compared with the AFB1 intoxicated group. Co-administration of MLEMC improved the histological characteristics of the hepatocytes in contrast to the AFB1 treated group, which had mild to severe hepatocellular injuries including bile duct proliferation, bile duct hyperplasia, lymphoplasmacytic infiltrate and fibrosis. Extracts of M. caffra were beneficial in mitigating the hepatotoxic effects of AFB1 in rats by reducing the levels of liver enzymes and preventing hepatic injury.Keywords
This publication has 29 references indexed in Scilit:
- Polyoxygenated cyclohexene derivatives from Monanthotaxis congoensisPhytochemistry, 2012
- Effects of Aflatoxin on Liver and Protective Effectiveness of Esterified Glucomannan in Merino RamsThe Scientific World Journal, 2012
- Inhibitory effects of quercetin on aflatoxin B1-induced hepatic damage in miceFood and Chemical Toxicology, 2010
- Metabolic intervention of aflatoxin B1 toxicity by curcuminJournal of Ethnopharmacology, 2010
- Comparative effects of curcumin and resveratrol on aflatoxin B1-induced liver injury in ratsArchives of Toxicology, 2010
- Aflatoxins: Detection, toxicity, and biosynthesisBiotechnology and Bioprocess Engineering, 2007
- Modulation of aflatoxin toxicity and biomarkers by lycopene in F344 ratsToxicology and Applied Pharmacology, 2007
- Aflatoxin B1-induced DNA damage and its repairCancer Letters, 2006
- Protective effect of food additives on aflatoxin-induced mutagenicity and hepatocarcinogenicityCancer Letters, 1997
- First enantiospecific syntheses of crotepoxide and iso-crotepoxide from (−)-quinic acidTetrahedron: Asymmetry, 1996