Best practices and tools for reporting reproducible fluorescence microscopy methods
- 7 June 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Methods
- Vol. 18 (12), 1463-1476
- https://doi.org/10.1038/s41592-021-01156-w
Abstract
Although fluorescence microscopy is ubiquitous in biomedical research, microscopy methods reporting is inconsistent and perhaps undervalued. We emphasize the importance of appropriate microscopy methods reporting and seek to educate researchers about how microscopy metadata impact data interpretation. We provide comprehensive guidelines and resources to enable accurate reporting for the most common fluorescence light microscopy modalities. We aim to improve microscopy reporting, thus improving the quality, rigor and reproducibility of image-based science.This publication has 60 references indexed in Scilit:
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