Quality Evaluation and Characterization of Fractions with Biological Activity from Ephedra Herb Extract and Ephedrine Alkaloids-Free Ephedra Herb Extract
Open Access
- 1 February 2020
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 68 (2), 140-149
- https://doi.org/10.1248/cpb.c19-00761
Abstract
Previously, we reported that the c-Met inhibitory effect of Ephedra Herb extract (EHE) is derived from ingredients besides ephedrine alkaloids. Moreover, analgesic and anti-influenza activities of EHE and ephedrine alkaloids-free Ephedra Herb extract (EFE) have been reported recently. In this study, we examined the fractions containing c-Met kinase inhibitory activity from EHE and the fractions with analgesic and anti-influenza activities from EFE, and elucidated the structural characteristics of the active fractions. Significant c-Met kinase activity was observed in 30, 40, and 50% methanol (MeOH) eluate fractions obtained from water extract of EHE using Diaion HP-20 column chromatography. Similarly, 20 and 40% MeOH, and MeOH eluate fractions obtained from water extract of EFE were found to display analgesic and anti-influenza activities. Reversed phase-HPLC analysis of the active fractions commonly showed broad peaks characteristic of high-molecular mass condensed tannin. The active fractions were analyzed using 13C-NMR and decomposition reactions; the deduced structures of active components were high-molecular mass condensed tannins, which were mainly procyanidin B-type and partly procyanidin A-type, including pyrogallol- and catechol-type flavan 3-ols as extension and terminal units. HPLC and gel permeation chromatography (GPC) analyses estimated that the ratio of pyrogallol- and catechol-type was approximately 9 : 2, and the weight-average molecular weight based on the polystyrene standard was >45000. Furthermore, GPC-based analysis was proposed as the quality evaluation method for high-molecular mass condensed tannin in EHE and EFE.Keywords
This publication has 21 references indexed in Scilit:
- Analgesic Effects of Ephedra Herb Extract, Ephedrine Alkaloids–Free Ephedra Herb Extract, Ephedrine, and Pseudoephedrine on Formalin-Induced PainBiological & Pharmaceutical Bulletin, 2019
- Ephedrine Alkaloids-Free Ephedra Herb Extract, EFE, Has No Adverse Effects Such as Excitation, Insomnia, and ArrhythmiasBiological & Pharmaceutical Bulletin, 2018
- Efficiently prepared ephedrine alkaloids-free Ephedra Herb extract: a putative marker and antiproliferative effectsJournal of Natural Medicines, 2016
- Ephedrine alkaloids-free Ephedra Herb extract: a safer alternative to ephedra with comparable analgesic, anticancer, and anti-influenza activitiesJournal of Natural Medicines, 2016
- Herbacetin, A Constituent of Ephedrae herba, Suppresses the HGF-Induced Motility of Human Breast Cancer MDA-MB-231 Cells by Inhibiting c-Met and Akt PhosphorylationPlanta Medica, 2013
- Characterization of Phenolic Constituents from Ephedra Herb ExtractMolecules, 2013
- Toxicological Risks of Chinese HerbsPlanta Medica, 2010
- Quality and safety of Chinese herbal medicines guided by a systems biology perspectiveJournal of Ethnopharmacology, 2009
- Inhibitory effect of Ephedrae herba, an oriental traditional medicine, on the growth of influenza A/PR/8 virus in MDCK cellsAntiviral Research, 1999
- Antiinflammatory principle of Ephedra herbs.CHEMICAL & PHARMACEUTICAL BULLETIN, 1980