Interaction of Plasmodium falciparum casein kinase 1 with components of host cell protein trafficking machinery
- 1 May 2020
- journal article
- research article
- Published by Wiley in IUBMB Life
- Vol. 72 (6), 1243-1249
- https://doi.org/10.1002/iub.2294
Abstract
A pool of Plasmodium falciparum casein kinase 1 (PfCK1) has been shown to localize to the host red blood cell (RBC) membrane and be secreted to the extracellular medium during trophozoite stage of development. We attempted to identify mechanisms for secretion of PfCK1 and its appearance on the RBC membrane. We found that two host proteins with established functions in membrane trafficking in higher eukaryotes, GTPase‐activating protein and Vps9 domain‐containing protein 1 (GAPVD1), and Sorting nexin 22, consistently co‐purify with PfCK1, suggesting that the parasite utilizes trafficking pathways previously thought to be inactive in RBCs. Furthermore, reciprocal immunoprecipitation experiments with GAPVD1 identified parasite proteins suggestive of a protein recycling pathway hitherto only described in higher eukaryotes. Thus, we have identified components of a trafficking pathway involving parasite proteins that act in concert with host proteins, and which we hypothesize mediates trafficking of PfCK1 to the RBC during infection.Keywords
This publication has 40 references indexed in Scilit:
- The human phosphatase interactome: An intricate family portraitFEBS Letters, 2012
- SNX3 controls Wingless/Wnt secretion through regulating retromer-dependent recycling of WntlessCell Research, 2011
- Evidence for prenylation-dependent targeting of a Ykt6 SNARE in Plasmodium falciparumMolecular and Biochemical Parasitology, 2011
- A Tissue-Specific Atlas of Mouse Protein Phosphorylation and ExpressionCell, 2010
- Breast cancer-specific mutations in CK1ε inhibit Wnt/β-catenin and activate the Wnt/Rac1/JNK and NFAT pathways to decrease cell adhesion and promote cell migrationBreast Cancer Research, 2010
- Mammalian Casein Kinase 1α and Its Leishmanial Ortholog Regulate Stability of IFNAR1 and Type I Interferon SignalingMolecular and Cellular Biology, 2009
- MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantificationNature Biotechnology, 2008
- A quantitative atlas of mitotic phosphorylationProceedings of the National Academy of Sciences of the United States of America, 2008
- Insulin Stimulates Phosphatidylinositol 3-Phosphate Production via the Activation of Rab5Molecular Biology of the Cell, 2008
- Gapex-5, a Rab31 Guanine Nucleotide Exchange Factor that Regulates Glut4 Trafficking in AdipocytesCell Metabolism, 2007