Label-free three-dimensional observations and quantitative characterisation of on-chip vasculogenesis using optical diffraction tomography
- 6 January 2021
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Lab on a Chip
- Vol. 21 (3), 494-501
- https://doi.org/10.1039/d0lc01061h
Abstract
Label-free, three-dimensional (3D) quantitative observations of on-chip vasculogenesis were achieved using optical diffraction tomography. Exploiting 3D refractive index maps as an intrinsic imaging contrast, the vascular structures, multicellular activities, and subcellular organelles of endothelial cells were imaged and analysed throughout vasculogenesis to characterise mature vascular networks without exogenous labelling.Keywords
Funding Information
- National Research Foundation of Korea (2015R1A3A2066550, 2017M3C1A3013923, 2018K000396, 2020R1A2C1100471, 2020R1A5A8018367)
- KAIST
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