Profiling of RNA N-6-Methyladenosine Methylation Reveals the Critical Role of m(6)A in Chicken Adipose Deposition
Open Access
- 4 February 2021
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Cell and Developmental Biology
Abstract
One of the main objectives of broiler breeding is to prevent excessive abdominal adipose deposition. The role of RNA modification in adipose deposition is not clear. This study was aimed to map m(6)A modification landscape in chicken adipose tissue. MeRIP-seq was performed to compare the differences in m(6)A methylation pattern between fat and lean broilers. We found that start codons, stop codons, coding regions, and 3 '-untranslated regions were generally enriched for m(6)A peaks. The high m(6)A methylated genes (fat birds vs. lean birds) were primarily associated with fatty acid biosynthesis and fatty acid metabolism, while the low m(6)A methylated genes were mainly involved in processes associated with development. Furthermore, we found that the mRNA levels of many genes may be regulated by m(6)A modification. This is the first comprehensive characterization of m(6)A patterns in the chicken adipose transcriptome, and provides a basis for studying the role of m(6)A modification in fat deposition.Keywords
Funding Information
- National Natural Science Foundation of China
- Agriculture Research System of China
This publication has 66 references indexed in Scilit:
- Comprehensive Analysis of mRNA Methylation Reveals Enrichment in 3′ UTRs and near Stop CodonsCell, 2012
- Comparison of adipose tissue cellularity in chicken lines divergently selected for fatnessPoultry Science, 2011
- DREME: motif discovery in transcription factor ChIP-seq dataBioinformatics, 2011
- Extensive chromatin remodelling and establishment of transcription factor ‘hotspots’ during early adipogenesisThe EMBO Journal, 2011
- Histone H3K27 methyltransferase Ezh2 represses Wnt genes to facilitate adipogenesisProceedings of the National Academy of Sciences of the United States of America, 2010
- Model-based Analysis of ChIP-Seq (MACS)Genome Biology, 2008
- A role of lipin in human obesity and insulin resistance: relation to adipocyte glucose transport and GLUT4 expressionJournal of Lipid Research, 2007
- Adipocytes as regulators of energy balance and glucose homeostasisNature, 2006
- Transcriptional control of adipocyte formationCell Metabolism, 2006
- The methylated constituents of L cell messenger RNA: Evidence for an unusual cluster at the 5′ terminusCell, 1975