Expression of ribosomal protection protein RppA is regulated by a ribosome‐dependent ribo‐regulator and two mistranslation products
Open Access
- 26 June 2020
- journal article
- research article
- Published by Wiley in Environmental Microbiology
- Vol. 23 (2), 696-712
- https://doi.org/10.1111/1462-2920.15143
Abstract
Gene expression is tightly controlled by transcription factors and RNA regulatory elements, including trans ‐acting small RNAs, cis ‐regulatory riboswitches, and ribosome‐dependent ribo‐regulators. In the present study, we demonstrated that a ribosome‐dependent ribo‐regulator and two mistranslation products co‐regulate rppA (encoding a ribosomal protection protein) expression in Bacillus thuringiensis BMB171. The leader RNA of the rppA gene controls rppA expression via translation of leader ORF1 resident in its sequence. In the presence of Chloramphenicol, a +1 frameshift product (ORF2) and a stop codon readthrough product (ORF3) of ORF1 emerged. ORF3 exerted a negative effect on rppA expression. By contrast, the ORF2 promoted rppA expression. The regulation mode identified in the present study will lead to a deeper understanding of bacterial gene expression.Keywords
Funding Information
- National Basic Research Program of China (2017YFD0200400, 2018YFA0900102)
- National Natural Science Foundation of China (31670081)
This publication has 48 references indexed in Scilit:
- HflXr, a homolog of a ribosome-splitting factor, mediates antibiotic resistanceProceedings of the National Academy of Sciences of the United States of America, 2018
- Errors during Gene Expression: Single-Cell Heterogeneity, Stress Resistance, and Microbe-Host InteractionsmBio, 2018
- Structural basis for antibiotic resistance mediated by the Bacillus subtilis ABCF ATPase VmlRProceedings of the National Academy of Sciences of the United States of America, 2018
- Heterogeneity of Stop Codon Readthrough in Single Bacterial Cells and Implications for Population FitnessMolecular Cell, 2017
- Roles of Regulatory RNAs for Antibiotic Resistance in Bacteria and Their Potential Value as Novel Drug TargetsFrontiers in Microbiology, 2017
- Regulation of antibiotic-resistance by non-coding RNAs in bacteriaCurrent Opinion in Microbiology, 2017
- Term-seq reveals abundant ribo-regulation of antibiotics resistance in bacteriaScience, 2016
- Protein mistranslation protects bacteria against oxidative stressNucleic Acids Research, 2015
- Molecular mechanisms of antibiotic resistanceNature Reviews Microbiology, 2014
- SsrA‐mediated protein tagging in the presence of miscoding drugs and its physiological role in Escherichia coliGenes to Cells, 2002