Halo-fluorescein for photodynamic bacteria inactivation in extremely acidic conditions
Open Access
- 22 January 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Communications
- Vol. 12 (1), 1-12
- https://doi.org/10.1038/s41467-020-20869-8
Abstract
Aciduric bacteria that can survive in extremely acidic conditions (pH < 4.0) are challenging to the current antimicrobial approaches, including antibiotics and photodynamic bacteria inactivation (PDI). Here, we communicate a photosensitizer design concept of halogenation of fluorescein for extremely acidic PDI. Upon halogenation, the well-known spirocyclization that controls the absorption of fluorescein shifts to the acidic pH range. Meanwhile, the heavy atom effect of halogens boosts the generation of singlet oxygen. Accordingly, several photosensitizers that could work at even pH < 2.0 were discovered for a broad band of aciduric bacteria families, with half maximal inhibitory concentrations (IC50) lower than 1.1 μM. Since one of the discovered photosensitizers is an FDA-approved food additive (2’,4’,5’,7’-tetraiodofluorescein, TIF), successful bacteria growth inhibition in acidic beverages was demonstrated, with greatly extended shelf life from 2 days to ~15 days. Besides, the in vivo PDI of Candidiasis with TIF under extremely acidic condition was also demonstrated.This publication has 49 references indexed in Scilit:
- Mouse model of oropharyngeal candidiasisNature Protocols, 2012
- Alicyclobacillus spoilage and isolation – A reviewFood Microbiology, 2011
- LysK, the enzyme lysing Staphylococcus aureus cells: Specific kinetic features and approaches towards stabilizationBiochimie, 2010
- Photoactive Hybrid Nanomaterial for Targeting, Labeling, and Killing Antibiotic‐Resistant BacteriaAngewandte Chemie, 2009
- The role of surfaces in catheter-associated infectionsChemical Society Reviews, 2009
- Effect of storage on the content of polyphenols, vitamin C and the antioxidant activity of orange juicesJournal of Food Composition and Analysis, 2007
- Symposium‐in‐Print: Singlet Oxygen Invited ReviewPhotochemistry and Photobiology, 2006
- Alicyclobacillus acidiphilus sp. nov., a novel thermo-acidophilic, omega-alicyclic fatty acid-containing bacterium isolated from acidic beveragesInternational Journal of Systematic and Evolutionary Microbiology, 2002
- A Compilation of Singlet Oxygen Yields from Biologically Relevant MoleculesPhotochemistry and Photobiology, 1999
- Photochemical heavy-atom effectsAccounts of Chemical Research, 1978