Supplementation with Proline Improves Haemato-Biochemical and Reproductive Indicators in Male Rabbits Affected by Environmental Heat-Stress
Open Access
- 1 February 2021
- Vol. 11 (2), 373
- https://doi.org/10.3390/ani11020373
Abstract
Twenty-four adult rabbit bucks (n = 6 per treatment) were fed a basal diet supplemented with 0 (control), 50, 100, and 150 mg proline/kg dry matter (DM) diet for 12 weeks to determine possible usefulness for alleviating the negative impact of environmental heat stress on redox status, haemato-biochaemical attributes and semen quality. There were significant dose–response effects, with increments in levels of dietary proline (LDP) quadratically improving red blood cell counts (p = 0.017), rectal temperature (p = 0.009), and respiratory rate (p < 0.001). Increasing LDP cubically affected superoxide dismutase activity in blood plasma (p = 0.012) and total antioxidant capacity in both blood and seminal plasma (p < 0.001 and p = 0.006, respectively). The optimal response was observed at 30 and 80 mg proline/kg DM for blood and seminal plasma, respectively. With regards to homeostasis indexes, increments in LDP cubically modified blood plasma concentrations of total protein (p = 0.002) and albumin (p < 0.001), with an optimal response found at 70 mg proline/kg DM. A linear relationship (p = 0.005) was also observed between LDP and blood plasma glucose concentrations, with the optimal response being found at 100 mg proline/kg DM. Increasing LDP also showed positive effects on reproductive traits, with quadratic increases in blood plasma testosterone and cortisol concentrations (p < 0.001; optimal responses at 50 and 60 mg proline/kg DM, respectively), a positive linear relationship with in libido, ejaculate volume, sperm concentration and total sperm count (p < 0.001 for all; optimal responses observed at 100 mg proline/kg DM) and a quadratic increase in total functional sperm fraction (p < 0.001; optimal response at 70 mg proline mg/kg DM). Hence, the optimal positive effects of dietary proline supplementation on redox status, blood metabolites, and reproductive traits of rabbit bucks may be achieved at 50–100 mg/kg DM.This publication has 39 references indexed in Scilit:
- Proline Mechanisms of Stress SurvivalAntioxidants and Redox Signaling, 2013
- Arginine Can Be Synthesized from Enteral Proline in Healthy Adult HumansJournal of Nutrition, 2011
- Amino acids stimulate Akt phosphorylation, and reduce IL‐8 production and NF‐κB activity in HepG2 liver cellsMolecular Nutrition & Food Research, 2010
- Proline Metabolism and Microenvironmental StressAnnual Review of Nutrition, 2010
- Amino acids: metabolism, functions, and nutritionAmino Acids, 2009
- Amino acids and gaseous signalingAmino Acids, 2009
- Reproductive and productive performance of rabbit does submitted to an oral glucose supplementationAnimal, 2009
- Growth control via TOR kinase signaling, an intracellular sensor of amino acid and energy availability, with crosstalk potential to proline metabolismAmino Acids, 2008
- FNDC3A is required for adhesion between spermatids and Sertoli cellsDevelopmental Biology, 2006
- Molecular cloning of the bovine blood-brain barrier glucose transporter cDNA and demonstration of phylogenetic conservation of the 5′-untranslated regionMolecular and Cellular Neuroscience, 1990