Sarcosine Prostate Cancer Biomarker Detection by Controlling Oxygen in NiOx Membrane on Vertical Silicon Nanowires in Electrolyte-Insulator-Nanowire Structure
- 16 June 2020
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 92 (12), 8064-8071
- https://doi.org/10.1021/acs.analchem.9b04745
Abstract
Sarcosine prostate cancer biomarker with the low concentration of 1 pM has been detected by controlling oxygen from 1 to 15 sccm in a NiOx membrane on chemically etched vertical Si nanowires (SiNWs) in an electrolyte-insulator-nanowire (EIN) structure. The vertical Si nanowires with approximately 17 mu m length and polycrystalline NiOx membrane are observed by both field-emission scanning electron microscope (FE-SEM) and high-resolution transmission electron microscope (HRTEM) images, respectively. The optimized NiOx membrane with oxygen content of 4 sccm on planar SiOx/Si substrate shows good pH sensitivity of approximately 50 mV/pH, low hysteresis of 3.4 mV, and low drift rate of 2.4 mV/h as compared to other oxygen content membranes of 1, 10, and 15 sccm. Further, uric acid with the concentration of 0.1 mu M is detected directly by using the optimized NiOx membrane. In addition, repeatable H2O2 sensing with the low concentration of 10 mu M as well as prostate cancer biomarker is detected, which is owing to the reduction-oxidation phenomena of the NiOx membranes. The sensing mechanism is owing to the Ni2+/Ni3+ oxidation states of the NiOx membrane, which is confirmed by X-ray photoelectron spectroscopy. The optimized NiOx membrane on vertical Si nanowire in the EIN structure shows a good drift rate of 3.84 mV/h and sarcosine detection with improvement of approximately 1000 times as compared to the planar Si in an electrolyte-insulator-semiconductor (EIS) structure. This sensor paves a way to detect early-stage diagnosis of prostate cancer rapidly in the near future.Funding Information
- Chang Gung Memorial Hospital, Linkou (CMRPD2E0091)
- Ministry of Science and Technology, Taiwan (MOST-105-2221-E-182-002, MOST-106-2221-E-182-075)
This publication has 67 references indexed in Scilit:
- Scalable cross-point resistive switching memory and mechanism through an understanding of H2O2/glucose sensing using an IrOx/Al2O3/W structurePhysical Chemistry Chemical Physics, 2017
- Direct electrochemistry of cytochrome c immobilized on a graphene oxide–carbon nanotube composite for picomolar detection of hydrogen peroxideRSC Advances, 2014
- Colorimetric determination of sarcosine in urine samples of prostatic carcinoma by mimic enzyme palladium nanoparticlesAnalytica Chimica Acta, 2014
- Determination of common urine substances as an assay for improving prostate carcinoma diagnosticsOncology Reports, 2014
- Sensitive determination of the potential biomarker sarcosine for prostate cancer by LC-MS withN,N′-dicyclohexylcarbodiimide derivatizationJournal of Separation Science, 2013
- The Role of Sarcosine Metabolism in Prostate Cancer ProgressionNeoplasia, 2013
- Serum sarcosine increases the accuracy of prostate cancer detection in patients with total serum PSA less than 4.0 ng/mlThe Prostate, 2012
- Metabolomic profiles delineate potential role for sarcosine in prostate cancer progressionNature, 2009
- Tumor target prostate specific membrane antigen (PSMA) and its regulation in prostate cancerJournal of Cellular Biochemistry, 2003
- Comparison of Digital Rectal Examination and Serum Prostate Specific Antigen in the Early Detection of Prostate Cancer: Results of a Multicenter Clinical Trial of 6,630 MenJournal of Urology, 1994