Fine mapping of the qHTB1-1QTL, which confers heat tolerance at the booting stage, using an Oryza rufipogon Griff. introgression line
- 27 January 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Theoretical and Applied Genetics
- Vol. 133 (4), 1161-1175
- https://doi.org/10.1007/s00122-020-03539-7
Abstract
Key message The qHTB1-1 QTL, controlling heat tolerance at the booting stage in rice, was fine mapped to a 47.1 kb region containing eight candidate genes. Two positional candidate genes showed significant changes in expression levels under heat stress. Abstract High-temperature stress at the booting stage has the potential to significantly limit rice production. An interspecific advanced backcrossed population between the Oryza sativa L. cultivar R53 and the wild Oryza rufipogon Griff accession HHT4 was used as the source material to develop a set of chromosome segment introgression lines to elucidate the genetic mechanism of the qHTB1-1 QTL in heat tolerance. A single-chromosome-segment introgression line, IL01-15, was used to develop secondary populations for the mapping of qHTB1-1 on chromosome 1 for heat tolerance at the booting stage. Using the BC5F2, BC5F3, and BC5F4 populations, we first confirmed qHTB1-1 and validated the phenotypic effect. The qHTB1-1 QTL explained 13.1%, 16.9%, and 17.8% of the phenotypic variance observed in the BC5F2, BC5F3, and BC5F4 generations, respectively. Using homozygous recombinants screened from larger BC6F2 and BC6F3 populations, qHTB1-1 was fine mapped within a 47.1 kb region between markers RM11633 and RM11642. Eight putative predicted genes were annotated in the region, and six genes were predicted to encode expressed proteins. The expression patterns of these six genes demonstrated that LOC_Os01g53160 and LOC_Os01g53220 were highly induced by heat stress in IL01-15 compared to R53. Sequence comparison of the gene-coding regions of LOC_Os01g53160 and LOC_Os01g53220 between R53 and IL01-15 revealed one synonymous and two nonsynonymous SNPs in exons, respectively. Our results provide a basis for identifying the genes underlying qHTB1-1 and indicate that markers linked to the qHTB1-1 locus can be used to improve the heat tolerance of rice at the booting stage by marker-assisted selection.Funding Information
- The National Natural Science Foundation of China (31560382)
- the Natural Science Foundation of Jiangxi Province Distinguished Young Scholars (2018ACB21026)
- the Natural Science Foundation of Jiangxi Province (20151BAB214013, 2017BAB204020)
- Key Research and Development Program of Jiangxi Province (20161BBF60129)
- the Key Laboratory Project of Jiangxi Province (20181BCD40010)
- Jiangxi Special Fund for Agro-scientific Research in the Collaborative Innovation, China (JXXTCXFY201902)
- the National Key R&D Program of China, China (2017YFD 0301601, 2018YFD0301103)
This publication has 44 references indexed in Scilit:
- Genetic Analysis of Cold Tolerance at Seedling Stage and Heat Tolerance at Anthesis in Rice (Oryza sativa L.)Journal of Integrative Agriculture, 2012
- Quantitative trait loci associated with seed set under high temperature stress at the flowering stage in rice (Oryza sativa L.)Euphytica, 2010
- Functionalisation of nanoparticles for biomedical applicationsNano Today, 2010
- Physiological and proteomic approaches to address heat tolerance during anthesis in rice (Oryza sativa L.)Journal of Experimental Botany, 2009
- Heat shock factor gene family in rice: Genomic organization and transcript expression profiling in response to high temperature, low temperature and oxidative stressesPlant Physiology and Biochemistry, 2009
- Phenotyping Parents of Mapping Populations of Rice for Heat Tolerance during AnthesisCrop Science, 2008
- Impact of Humidity on Temperature‐Induced Grain Sterility in Rice (Oryza sativa L)Journal of Agronomy and Crop Science, 2008
- Multiple functions ofDrosophilaheat shock transcription factorin vivoThe EMBO Journal, 1997
- High Temperature-Induced Spikelet Sterility of Japonica Rice at Flowering in Relation to Air Temperature, Humidity and Wind Velocity Conditions.Japanese Journal of Crop Science, 1997
- MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populationsGenomics, 1987