Abundance of conserved CRISPR-Cas9 target sites within the highly polymorphic genomes of Anopheles and Aedes mosquitoes
Open Access
- 18 March 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Communications
- Vol. 11 (1), 1-6
- https://doi.org/10.1038/s41467-020-15204-0
Abstract
A number of recent papers report that standing genetic variation in natural populations includes ubiquitous polymorphisms within target sites for Cas9-based gene drive (CGD) and that these “drive resistant alleles” (DRA) preclude the successful application of CGD for managing these populations. Here we report the results of a survey of 1280 genomes of the mosquitoes Anopheles gambiae, An. coluzzii, and Aedes aegypti in which we determine that ~90% of all protein-encoding CGD target genes in natural populations include at least one target site with no DRAs at a frequency of ≥1.0%. We conclude that the abundance of conserved target sites in mosquito genomes and the inherent flexibility in CGD design obviates the concern that DRAs present in the standing genetic variation of mosquito populations will be detrimental to the deployment of this technology for population modification strategies.This publication has 41 references indexed in Scilit:
- Development and Applications of CRISPR-Cas9 for Genome EngineeringCell, 2014
- CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editingNucleic Acids Research, 2014
- Trimmomatic: a flexible trimmer for Illumina sequence dataBioinformatics, 2014
- Differential effects of inbreeding and selection on male reproductive phenotype associated with the colonization and laboratory maintenance of Anopheles gambiaeMalaria Journal, 2014
- Genome engineering using the CRISPR-Cas9 systemNature Protocols, 2013
- Multiplex Genome Engineering Using CRISPR/Cas SystemsScience, 2013
- The Genome Analysis Toolkit: A MapReduce framework for analyzing next-generation DNA sequencing dataGenome Research, 2010
- Anopheles mosquitoes: not just flying malaria vectors… especially in the fieldTrends in Parasitology, 2009
- CRISPR Provides Acquired Resistance Against Viruses in ProkaryotesScience, 2007
- Microsatellite DNA Polymorphism and Heterozygosity Among Field and Laboratory Populations ofAnopheles gambiaes.s. (Diptera: Culicidae)Journal of Medical Entomology, 2001