The objective of the work was to identify and describe the operational sanitary procedures (OSP) applied in the slaughterhouse of the Pantanal alligator, as well as the possible corrective actions for non-conformities that may be found during the process, in order to reduce or eliminate cross-contamination. Once the alligator slaughter production flowchart was known, it was possible to identify six control points: bleeding, skinning, evisceration, toilet, washing the carcasses and cooling. The OSP refers to a preventive program, as well as the other self-control programs, which must be executed based on preventive measures, thus avoiding possible non-conformities.