Host-specific differences in the response of cultured macrophages to Campylobacter jejuni capsule and O-methyl phosphoramidate mutants
Open Access
- 9 January 2018
- journal article
- research article
- Published by Springer Science and Business Media LLC in Veterinary Research
- Vol. 49 (1), 1-10
- https://doi.org/10.1186/s13567-017-0501-y
Abstract
Campylobacter jejuni is the leading cause of bacterial food-borne gastroenteritis worldwide and human infections are frequently associated with handling and consumption of contaminated poultry. The polysaccharide capsule of C. jejuni plays important roles in colonisation of the chicken gut, invasion of epithelial cells and serum resistance and is subject to modification with O-methyl phosphoramidate (MeOPN) in most strains. In this study, the cytokine responses of mouse bone marrow-derived macrophages (mBMMs), chicken bone marrow-derived macrophages (chBMMs) and human monocyte-derived macrophages (hMDMs) were measured following infection with C. jejuni 11168H wild-type (WT) or isogenic mutants lacking either the capsule (Δcj1439) or its MeOPN modification (Δcj1417). Consistent with previous observations using murine bone marrow-derived dendritic cells, mutants lacking the capsule or MeOPN elicited enhanced transcription of IL-6 and IL-10 in mBMMs compared to wild-type C. jejuni. However, the lack of capsule and MeOPN did not alter IL-6 and IL-10 expression in chBMMs and hMDMs compared to C. jejuni WT. Phagocytosis assays showed the acapsular mutant was not impaired in uptake or net intracellular survival after phagocytosis in both chicken and human macrophages; however, the phagocytosis of the MeOPN mutant was significantly decreased in both chicken and human macrophages. In conclusion, differences in the response of macrophages of varying host origin to Campylobacter were detected. The absence of MeOPN modification on the capsule of C. jejuni did not alter the levels of innate cytokine expression in both chicken and human macrophages compared to the 11168H WT, but affected phagocytosis by host macrophages.Keywords
Funding Information
- Biotechnology and Biological Sciences Research Council (BB/J004227/1, BB/J004219/1, BB/P013740/1)
- Scottish Government
This publication has 49 references indexed in Scilit:
- Longitudinal study of infectious intestinal disease in the UK (IID2 study): incidence in the community and presenting to general practiceGut, 2011
- Altered Linkage of Hydroxyacyl Chains in Lipid A of Campylobacter jejuni Reduces TLR4 Activation and Antimicrobial ResistanceOnline Journal of Public Health Informatics, 2010
- Activation of Human and Chicken Toll-Like Receptors by Campylobacter sppInfection and Immunity, 2010
- Campylobacter jejuni -Induced Activation of Dendritic Cells Involves Cooperative Signaling through Toll-Like Receptor 4 (TLR4)-MyD88 and TLR4-TRIF AxesInfection and Immunity, 2009
- Colony-stimulating factor-1 (CSF-1) delivers a proatherogenic signal to human macrophagesJournal of Leukocyte Biology, 2008
- Reactive Nitrogen Species Contribute to Innate Host Defense againstCampylobacter jejuniInfection and Immunity, 2008
- Cytokine responses in primary chicken embryo intestinal cells infected with Campylobacter jejuni strains of human and chicken origin and the expression of bacterial virulence-associated genesBMC Microbiology, 2008
- Myd88-dependent positioning of Ptgs2-expressing stromal cells maintains colonic epithelial proliferation during injuryJCI Insight, 2007
- Campylobacter jejuniInduces Maturation and Cytokine Production in Human Dendritic CellsInfection and Immunity, 2006
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001