24-epibrassinolide induces protection against waterlogging and alleviates impacts on the root structures, photosynthetic machinery and biomass in soybean
- 1 November 2020
- journal article
- research article
- Published by Informa UK Limited in Plant Signaling & Behavior
- Vol. 15 (11)
- https://doi.org/10.1080/15592324.2020.1805885
Abstract
Plants exhibit several restrictions under waterlogging conditions, including stomatal limitations, negative impacts on gas exchange, lower nutrient uptake and reduced growth. 24-epibrassinolide (EBR) is a polyhydroxylated steroid, with the advantages to be a natural and biodegradable molecule, presenting beneficial roles in metabolic and physiological processes. The aim of this research is to investigate whether EBR can protect soybean plants against damage caused by waterlogging and evaluate the responses associated with the root and leaf anatomy, photosynthetic machinery and biomass. This study used a completely randomized factorial design with two water conditions (control and waterlogging) and three concentrations of 24-epibrassinolide (0, 5 and 10 nM EBR). This steroid stimulated the activities of enzymes linked to the antioxidant system and resulted in minor damage to the chloroplast membranes. EBR maximized the efficiency of photosystem II and improved the gas exchange, which was explained by the higher density and index of the stomata in addition to the increased chlorophyll content and electron transport rate. In root structures, EBR mitigated the impact of waterlogging on vascular cylinder and metaxilem, suggesting maintenance and functions of these structures in plants stressed.Keywords
Funding Information
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Fundação Amazônia de Amparo a Estudos e Pesquisas
- Universidade Federal de Uberlândia
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Programa de Pós-Graduação em Agronomia
This publication has 48 references indexed in Scilit:
- Changes in leaf anatomy and photosynthesis of micropropagated banana plantlets under different silicon sourcesScientia Horticulturae, 2013
- Role of brassinosteroids in alleviation of phenanthrene–cadmium co-contamination-induced photosynthetic inhibition and oxidative stress in tomatoJournal of Experimental Botany, 2012
- Brassinosteroid regulates stomatal development by GSK3-mediated inhibition of a MAPK pathwayNature, 2012
- Alleviation of waterlogging stress in upland cotton (Gossypium hirsutum L.) by exogenous application of potassium in soil and as a foliar sprayCrop and Pasture Science, 2011
- Reactive Oxygen Species Are Involved in Brassinosteroid-Induced Stress Tolerance in CucumberPlant Physiology, 2009
- Waterlogging induced oxidative stress and antioxidant activity in pigeonpea genotypesBiologia plantarum, 2009
- Influence of brassinosteroids on initiation of the root gravitropic response in Pisum sativum seedlingsBiologia plantarum, 2006
- Magnesium Deficiency and High Light Intensity Enhance Activities of Superoxide Dismutase, Ascorbate Peroxidase, and Glutathione Reductase in Bean LeavesPlant Physiology, 1992
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Polychromatic staining of plant cell walls by toluidine blue OProtoplasma, 1964