Reactivation of the pluripotency program precedes formation of the cranial neural crest
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- 3 February 2021
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 371 (6529), 586-+
- https://doi.org/10.1126/science.abb4776
Abstract
During development, cells progress from a pluripotent state to a more restricted fate within a particular germ layer. However, cranial neural crest cells (CNCCs), a transient cell population that generates most of the craniofacial skeleton, have much broader differentiation potential than their ectodermal lineage of origin. Here, we identify a neuroepithelial precursor population characterized by expression of canonical pluripotency transcription factors that gives rise to CNCCs and is essential for craniofacial development. Pluripotency factor Oct4 is transiently reactivated in CNCCs and is required for the subsequent formation of ectomesenchyme. Furthermore, open chromatin landscapes of Oct4(+) CNCC precursors resemble those of epiblast stem cells, with additional features suggestive of priming for mesenchymal programs. We propose that CNCCs expand their developmental potential through a transient reacquisition of molecular signatures of pluripotency.Funding Information
- Howard Hughes Medical Institute
- NIH Office of the Director (R35 GM131757)
- European Molecular Biology Organization (ALTF 275-2015)
- Virginia and D.K. Ludwig Fund for Cancer Research
- Polish National Science Center (ETIUDA 7 2019/32/T/NZ3/00370)
- University of Warsaw Integrated Development Programme
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