Nuclear SRP9/SRP14 heterodimer transcriptionally regulates 7SL and BC200 RNA expression
Open Access
- 8 May 2023
- journal article
- research article
- Published by Cold Spring Harbor Laboratory in RNA
- Vol. 29 (8), 1185-1200
- https://doi.org/10.1261/rna.079649.123
Abstract
The SRP9/SRP14 heterodimer is a central component of signal recognition particle (SRP) RNA (7SL) processing and Alu retrotransposition. In this study, we sought to establish the role of nuclear SRP9/SRP14 in the transcriptional regulation of 7SL and BC200 RNA. 7SL and BC200 RNA steady state levels, rate of decay, and transcriptional activity were evaluated under SRP9/SRP14 knockdown conditions. Immunofluorescent imaging, and subcellular fractionation of MCF-7 cells, revealed a distinct nuclear localization for SRP9/SRP14. The relationship between this localization and transcriptional activity at 7SL and BC200 genes was also examined. These findings demonstrate a novel nuclear function of SRP9/SRP14 establishing that this heterodimer transcriptionally regulates 7SL and BC200 RNA expression. We describe a model in which SRP9/SRP14 co-transcriptionally regulate 7SL and BC200 RNA expression. Our model is also a plausible pathway for regulating Alu RNA transcription and is consistent with the hypothesized roles of SRP9/SRP14 transporting 7SL RNA into the nucleolus for post-transcriptional processing, and trafficking of Alu RNA for retrotransposition.Keywords
Funding Information
- Canadian Institutes of Health Research Project Grant (427781)
- Natural Sciences and Engineering Research Council of Canada CGS-D
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