Scent dog identification of SARS-CoV-2 infections, similar across different body fluids
Preprint
- 5 March 2021
- preprint
- research article
- Published by Cold Spring Harbor Laboratory
Abstract
Background The main strategy to contain the current SARS-CoV-2 pandemic remains to implement a comprehensive testing, tracing and quarantining strategy until vaccination of the population is adequate. Methods Ten dogs were trained to detect SARS-CoV-2 infections in beta-propiolactone inactivated saliva samples. The subsequent cognitive transfer performance for the recognition of non-inactivated samples were tested on saliva, urine, and sweat in a randomised, double-blind controlled study. Results Dogs were tested on a total of 5242 randomised sample presentations. Dogs detected non-inactivated saliva samples with a diagnostic sensitivity of 84% and specificity of 95%. In a subsequent experiment to compare the scent recognition between the three non-inactivated body fluids, diagnostic sensitivity and specificity were 95% and 98% for urine, 91% and 94% for sweat, 82%, and 96% for saliva respectively. Conclusions The scent cognitive transfer performance between inactivated and non-inactivated samples as well as between different sample materials indicates that global, specific SARS-CoV-2-associated volatile compounds are released across different body secretions, independently from the patient’s symptoms. Funding The project was funded as a special research project of the German Armed Forces. The funding source DZIF-Fasttrack 1.921 provided us with means for biosampling.Keywords
This publication has 30 references indexed in Scilit:
- Biased efficacy estimates in phase-III dengue vaccine trials due to heterogeneous exposure and differential detectability of primary infections across trial armsPLOS ONE, 2019
- Biomedical Scent Detection Dogs: Would They Pass as a Health Technology?Pet Behaviour Science, 2018
- Detecting Staphylococcus aureus in milk from dairy cows using sniffer dogsJournal of Dairy Science, 2018
- Sensitivity, Specificity, and Predictive Values: Foundations, Pliabilities, and Pitfalls in Research and PracticeFrontiers in Public Health, 2017
- Antibody and B Cell Subset Perturbations in Human Immunodeficiency Virus-Uninfected Patients With CryptococcosisOpen Forum Infectious Diseases, 2017
- Canine Detection of the Volatilome: A Review of Implications for Pathogen and Disease DetectionFrontiers in Veterinary Science, 2016
- The human volatilome: volatile organic compounds (VOCs) in exhaled breath, skin emanations, urine, feces and salivaJournal of Breath Research, 2014
- Cellular Scent of Influenza Virus Infection.ChemBioChem, 2014
- A universal heterologous internal control system for duplex real-time RT-PCR assays used in a detection system for pestivirusesJournal of Virological Methods, 2006
- Diagnostic Accuracy of Canine Scent Detection in Early- and Late-Stage Lung and Breast CancersIntegrative Cancer Therapies, 2006