Solubilization and Stabilization of Membrane Proteins by Cycloalkane-Modified Amphiphilic Polymers
- 30 June 2020
- journal article
- research article
- Published by American Chemical Society (ACS) in Biomacromolecules
- Vol. 21 (8), 3459-3467
- https://doi.org/10.1021/acs.biomac.0c00929
Abstract
Membrane proteins need to be extracted from biological membranes and purified in their native state for most structural and functional in vitro investigations. Amphiphilic copolymers, such as amphipols (APols), have emerged as very useful alternatives to detergents for keeping membrane proteins water-soluble under their native form. However, classical APols, i.e. poly(acrylic acid) (PAA) derivatives, seldom enable direct membrane protein extraction. Poly(styrene-maleic anhydride) copolymers (SMAs), which bear aromatic rings as hydrophobic side groups, have been reported to be more effective extracting agents. In order to test the hypothesis of a role of cyclic hydrophobic moieties in membrane solubilization by copolymers, we have prepared PAA derivatives comprising cyclic rather than linear aliphatic side groups (CyclAPols). As references, APol A8-35, SMAs, and diisobutylene maleic acid (DIBMA) were compared with CyclAPols. Using as models the plasma membrane of Escherichia coli and the extraction-resistant purple membrane from Halobacterium salinarum, we show that CyclAPols combine the extraction efficiency of SMAs with the stabilization afforded to membrane proteins by classical APols such as A8 35.Keywords
This publication has 40 references indexed in Scilit:
- Mammalian plasma membrane proteins as potential biomarkers and drug targetsElectrophoresis, 2011
- Amphipols From A to ZAnnual Review of Biophysics, 2011
- Membrane Proteins Solubilized Intact in Lipid Containing Nanoparticles Bounded by Styrene Maleic Acid CopolymerJournal of the American Chemical Society, 2009
- Drugs and their molecular targets: an updated overviewFundamental & Clinical Pharmacology, 2008
- Flexible macromolecules attached to lipid bilayers: impact on fluidity, curvature, permeability and stability of the membranesSoft Matter, 2008
- Association of Octyl-Modified Poly(acrylic acid) onto Unilamellar Vesicles of Lipids and Kinetics of Vesicle DisruptionLangmuir, 2004
- Stabilization of membranes upon interaction of amphipathic polymers with membrane proteinsProtein Science, 2004
- Comparison of the Aggregation Behavior of Di- and Triblock Nonionic Amphiphiles with Linear and Cyclic Hydrophobic TailsLangmuir, 1997
- Micellar Properties of Sodium Cyclododecyl Sulfate in Aqueous SolutionLangmuir, 1996
- Influence of hydrocarbon chain branching on interfacial properties of sodium dodecyl sulfateLangmuir, 1992