JMY expression by Sertoli cells contributes to mediating spermatogenesis in mice
Open Access
- 11 April 2020
- journal article
- research article
- Published by Wiley in The FEBS Journal
- Vol. 287 (24), 5478-5497
- https://doi.org/10.1111/febs.15328
Abstract
Sertoli cells are crucial for spermatogenesis in the seminiferous epithelium because their actin cytoskeleton supports vesicular transport, cell junction formation, protein anchoring and spermiation. Here we show that a junction‐mediating and actin regulatory protein (JMY), affects the blood tissue barrier (BTB) function through remodeling of the Sertoli cell junctional integrity and it also contributes to controlling endocytic vesicle trafficking. These functions are critical for the maintenance of sperm fertility since loss of Sertoli cell‐specific Jmy function induced male subfertility in mice. Specifically, these mice have: a) impaired BTB integrity and spermatid adhesion in the seminiferous tubules; b) high incidence of sperm structural deformity; c) reduced sperm count and poor sperm motility. Moreover, the cytoskeletal integrity was compromised along with endocytic vesicular trafficking. These effects impaired junctional protein recycling and reduced Sertoli cell BTB junctional integrity. In addition, JMY interaction with actin binding protein candidates α‐actinin1 and Sorbs2 was related to JMY activity, and in turn actin cytoskeletal organization. In summary, JMY affects the control of spermatogenesis through the regulation of actin filament organization and endocytic vesicle trafficking in Sertoli cells.Keywords
Funding Information
- National Natural Science Foundation of China (81701503, 81571487, 81370752, 81971437)
This publication has 56 references indexed in Scilit:
- Adjudin disrupts spermatogenesis via the action of some unlikely partnersSpermatogenesis, 2011
- Actin-binding protein drebrin E is involved in junction dynamics during spermatogenesisSpermatogenesis, 2011
- Regulation of blood-testis barrier dynamics by desmosome, gap junction, hemidesmosome and polarity proteinsSpermatogenesis, 2011
- Differential effects of testosterone and TGF-β3 on endocytic vesicle-mediated protein trafficking events at the blood–testis barrierExperimental Cell Research, 2010
- WASP Family Proteins: Their Evolution and Its Physiological ImplicationsMolecular Biology of the Cell, 2010
- Restricted Arp3 expression in the testis prevents blood–testis barrier disruption during junction restructuring at spermatogenesisProceedings of the National Academy of Sciences of the United States of America, 2010
- A transcription co-factor integrates cell adhesion and motility with the p53 responseProceedings of the National Academy of Sciences of the United States of America, 2009
- Double JMY: making actin fastNature, 2009
- p53-cofactor JMY is a multifunctional actin nucleation factorNature, 2009
- Blood‐testis barrier dynamics are regulated by testosterone and cytokinesviatheir differential effects on the kinetics of protein endocytosis and recycling in Sertoli cellsThe FASEB Journal, 2008