Evaluation of anti-oxidant, anti-cholinesterase activity and dopamine levels of methanolic leaves extract of Solanum incanum in arsenic-induced neurodegeneration in Wistar rats

Abstract

The study investigated the anti-oxidant, anti-cholinesterase activity and dopamine levels of methanolic leaves extract Solanum incanum in arsenic-induced neurodegeneration. Animals were divided into four groups: A Control (maintained on food and water only), B treated with 300 mg/kg S. incanum, C treated with 20 mg/kg As2O3, and D treated with 300 mg/kg and then 20 mg/kg As2O3. Serum anti-oxidant activities were determined by measuring the Catalase (CAT) and Super-oxidase (SOD) activities using enzyme-linked immunosorbent assay (ELISA). Serum acetylcholinesterase inhibitory activity and dopamine levels were also quantified using ELISA. Prepared slides from the brain were dehydrated using a dry air oven in xylene for 30 minutes and later mounted on the microscope and viewed using oil immersion ×1000 magnification. The result of CAT and SOD assays showed significant decrease (P