Polydopamine-based molecular imprinting on silica-modified magnetic nanoparticles for recognition and separation of bovine hemoglobin
- 6 November 2012
- journal article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 138 (2), 651-658
- https://doi.org/10.1039/c2an36313e
Abstract
Surface molecular imprinting, especially on the surface of silica-modified magnetic nanoparticles, has been proposed as a promising strategy for protein recognition and separation. Inspired by the self-polymerization of dopamine, we synthesized a polydopamine-based molecular imprinted film coating on silica–Fe3O4 nanoparticles for recognition and separation of bovine hemoglobin (BHb). Magnetic molecularly imprinted nanoparticles (about 860 nm) possess a core–shell structure. Magnetic molecularly imprinted nanoparticles (MMIP) show a relatively high adsorption capacity (4.65 ± 0.38 mg g−1) and excellent selectivity towards BHb with a separation factor of 2.19. MMIP with high saturation magnetization (10.33 emu g−1) makes it easy to separate the target protein from solution by an external magnetic field. After three continuous adsorption and elution processes, the adsorption capacity of MMIP remained at 4.30 mg g−1. Our results suggest that MMIPs are suitable for the removal of high abundance of protein and the enrichment of low abundance of protein in proteomics.Keywords
This publication has 39 references indexed in Scilit:
- Preparation and adsorption selectivity of rutin molecularly imprinted polymersJournal of Applied Polymer Science, 2011
- Synthesis and characterization of molecularly imprinted polymer for controlled release of tramadolOpen Chemistry, 2010
- Mimicking biological delivery through feedback‐controlled drug release systems based on molecular imprintingAIChE Journal, 2009
- Molecularly imprinted solid-phase extraction for the determination of fenitrothion in tomatoesAnalytical and Bioanalytical Chemistry, 2008
- Detection of a specific DNA sequence by electrophoresis through a molecularly imprinted polymerBiomaterials, 2006
- A new molecularly imprinted polymer for selective extraction of cotinine from urine samples by solid-phase extractionAnalytical and Bioanalytical Chemistry, 2005
- Molecularly imprinted polymer-assisted sample clean-up of ochratoxin A from red wine: merits and limitationsJournal of Chromatography B, 2004
- Molecularly imprinted polymers as antibody and receptor mimics for assays, sensors and drug discoveryAnalytical and Bioanalytical Chemistry, 2004
- Clean-up of triazines in vegetable extracts by molecularly-imprinted solid-phase extraction using a propazine-imprinted polymerAnalytical and Bioanalytical Chemistry, 2003
- Food Analyses Using Molecularly Imprinted PolymersJournal of Agricultural and Food Chemistry, 2001